大鼠胰腺卵泡刺激素(β)及其受体的免疫荧光定位及原位杂交

Localization of FSHβ and its receptor in pancreas of rats

目的探讨大鼠胰腺组织中是否表达卵泡刺激素(FSH)及其受体(FSHR),为进一步研究FSH对胰腺的功能调节提供理论依据。方法采用单标记免疫荧光技术和原位杂交方法进行研究。结果大鼠胰岛大部分细胞及部分胰腺外分泌部细胞呈FSH蛋白和mRNA,FSHR蛋白和mRNA免疫荧光反应阳性,阳性物质分布于细胞质,细胞核呈阴性反应。结论大鼠胰腺细胞具有自身合成和分泌FSH及其受体的功能,说明FSH对胰腺细胞的作用可能通过旁分泌或自分泌的作用实现的。

Objective To study the localization of follicle stimulating hormone（FSH）, its receptor（FSHR） and their mRNA in pancreas of rats to provide theoretic evidence for further study of FSH function in pancreas. Methods Single-staining immunofluorescence and in situ hybridization methods were used. Results FSH protein and mRNA, and FSHR protein and mRNA immunofluorescenee positive staining was localized in most of rat islet cells and partial pancreas exocrine Cells, positive material distributed in the cytoplasm, not in nuclei. Conclusion Presentation of both FSH and FSHR in rat pancreas indicates that FSH may be involved in the functional regulation of pancreas through autocrine or paracrine activity.