碘甲烷对大鼠肝脏合成功能的影响

Study on the effect of methyl iodide on the synthetic function of rat liver

目的研究碘甲烷对大鼠肝脏合成功能的影响。方法将24只雄性SD大鼠随机分成4组,放入静式染毒罐中,实验组分别给予浓度为650 mg/m3、260 mg/m3、130 mg/m3的碘甲烷,每天吸入染毒4 h,连续染毒一周,对照组给予相同环境条件不染毒。染毒结束后对血清总蛋白(TP)、血清白蛋白(ALB)、血清球蛋白(GLO)、血清胆碱酯酶(CHE)、血清谷丙转氨酶(ALT)以及血清谷草转氨酶(AST)进行测定。结果随着碘甲烷浓度的升高,大鼠TP含量逐渐降低,高、中、低剂量组与对照组存在统计学差异,且高剂量组与中、低剂量组也存在统计学差异。ALB浓度随着碘甲烷浓度的升高逐渐降低,高、中、低剂量组与对照组存在统计学差异,且高剂量组与中、低剂量组也存在统计学差异。GLO含量随着碘甲烷浓度升高变化不大,各组间差异无统计学意义。CHE活力随着碘甲烷浓度升高逐渐降低,各组之间差异均有统计学意义。ALT含量随着碘甲烷浓度的升高逐渐升高,且实验组与对照组相比存在统计学差异,高剂量组与低剂量组比较差异有统计学意义。AST含量随着碘甲烷浓度的升高逐渐升高,实验组与对照组之间比较存在统计学差异,高、中剂量组与低剂量组比较,存在统计学差异。结论碘甲烷暴露对大鼠的肝脏合成功能有损害作用。

Objective To study the effects of methyl iodide to synthesis function of rat liver. Methods Twenty- four male SD rats were randomly divided into 4 groups, put them into the statie exposure tank. Three experimental groups were given the methyl iodide with concentrations of 650 mg/m3 , 260 mg/m3 and 130 mg/m3 respectively for a week period of time. The duration of daily inhalation was 4 hours. The control group was given the same environmental conditions without exposure. At the end of the exposure, the content of serum total protein （TP） , the content of serum albumin （ALB）, the content of serum globulin （GLO） and the activity of serum cholinesterase （CHE） were measured. Results With the increase of methyl iodide concentration, the content of TP of rats decreased. There was significant difference between experimental groups and control group, and there was also significant difference between high dose group with middle and low dose group. The content of ALB gradually decreased with the increase of the concentration of methyl iodide. There was significant difference between the experimental group and the control group, and there was also significant difference between high dose group with middle and low dose group. The content of GLO changed little with the increase of the concentration of methyl iodide, there was no significant difference between each group： The activity of CHE gradually decreased with the increase of the concentration of methyl iodide, and there was significant difference between each group. The content of ALT gradually increased with the increase of the concentration of methyl iodide. There was significant difference between the experimental group and the control group, and there was also significant difference between high dose group and low dose group. The content of AST gradually increased with the increase of the concentration of methyl iodide. There was significant difference between the experimental group and the control group, and there was also significant difference between high dose group with middle and low dose group. Conclusions Methyl iodide exposure may has the effect of synthesis function of rat liver.