山东省2010年手足口病患者和健康人来源的埃可病毒30型分离株的基因特征分析

Genetic analysis of Echovirus 30isolated from hand-foot-mouth disease cases and healthy controls from Shandong,China in 2010

目的了解2010年山东省来源于手足口病患者和健康人的埃可病毒30型（ECHO30）的基因特征，为制定肠道病毒感染的预防控制策略提供依据。方法采集手足口病患者和健康人群的粪便标本，接种于RD、Hep2细胞并分离病毒，阳性分离物采用分子生物学方法进行型别鉴定和VP1编码区基因的序列测定，对ECHO30毒株进行同源性分析，与GenBank检索到的其他ECHO30毒株构建基因系统进化树以分析ECHO30的基因特征。结果分别从手足口病患者和健康人的粪便标本中分离得到8株和10株ECHO30，同源性分析显示，两种来源的ECHO30在核苷酸水平上具有较高的同源性（92．7％～98．6％），在进化树上位于同一个分支，与2010年山东省临沂市分离株最近（96．7％～98．7％），与原型株同源性较低（79．6％～81．3％）。结论山东省2010年来源于手足口病患者和健康人的ECHO30亲缘关系较近，具有同一进化来源，健康人携带的ECHO30病毒可能成为手足口病的传染源。

Objective To characterize the Echovirus 30 （ECHO30） strains isolated in 2010 from hand-foot- mouth disease （HFMD） patients and healthy controls in Shandong, China. Methods Stool specimens were collected for virus isolation in RD and Hep-2 cells. Genotyping was done by RT-PCR of the VP1 region and se- quencing analysis. Homology and phylogenetic analysis were conducted. Results Eight ECHO30 strains were isolated from stool specimens of HFMD cases, and 10 from healthy controls. The isolates from these two pop- ulations shared high homology at nucleotide level （92.7%-98.6%）, and all isolates were grouped into one branch in the phylogenetic tree. The nucleotide homology with Linyi isolates are relatively high （96.7%- 98.7%） but lower with the prototype strain （79.6%-81.3%）. Conclusions The nucleotide sequences of ECHO30 strains isolated from HFMD patients and healthy controls share high homology, indicating the viru- ses from the two populations had the same origin, and healthy control carriers might be one of infectious sources of HFMD patients.