粤北南方红豆杉HPLC指纹图谱研究

Study on HPLC Fingerprint of Taxus chinensis var. mairei

目的:建立粤北南方红豆杉不同采收季节、不同采收部位的HPLC指纹图谱分析方法,为南方红豆杉的综合利用提供科学依据。方法:采用超临界CO2萃取技术提取南方红豆杉有效部位,按HPLC法建立指纹图谱,使用指纹谱图相似度软件进行评价分析。结果:建立了粤北南方红豆杉指纹图谱,对不同采收季节、不同采收部位的12批南方红豆杉样品提取物进行了指纹图谱分析,得出主要共有峰相对保留时间、相对峰面积作为南方红豆杉指纹图谱相关技术参数。结论:南方红豆杉指纹图谱可用于评价其药材质量。

Objective： To establish the HPLC fingerprint of Taxus chinensis var. mairei collected from different parts during different seasons and provide scientific basis for its comprehensive utilization. Methods ： Supercritical C02 extraction was used to extract the effective fraction, HPLC method to establish the fingerprint and similarity evaluation software to analyze the fingerprint chromatogram. Results： 12 batches of Taxus chinensis var. maire~ medicinal materials from different parts collected in different seasons were analyzed and HPLC fingerprint of Taxus chinensis var. mairei was established. Conclusion： The HPLC fingerprint can be used to evaluate the quality of Taxus chinensis vat. mairei medicinal materials.