三七总皂苷对肝纤维化大鼠细胞因子的影响

Effect of Panax notoginseng Saponins on Cytokines in Liver Fibrosis Rats

目的:观察三七总皂苷(PNS)对肝纤维化大鼠细胞因子产生的影响,探讨其抗肝纤维化的作用机制。方法:SD大鼠随机分成正常对照组、模型组、PNS(50、100、200 mg/kg)组和秋水仙碱(Col)组。除正常对照组外其余各组大鼠采用CCl4皮下注射的方法诱导肝纤维化模型,2次/w,连续18 w。于造模第9周起,给药组分别灌胃给予相应的受试药物,正常对照组和模型组给予等体积生理盐水,1次/d,疗程10 w。实验结束后,放射免疫法测定血清IL-1、IL-6、IL-10、TNF-α的含量;同时取固定部位肝脏组织,HE染色观察肝组织病理学改变,免疫组化技术测定肝组织中NF-κB、转化生长因子(TGF)-β蛋白的表达,RT-PCR技术测定肝组织中TGF-β、TNF-αmRNA表达。结果:与模型组比较,PNS(100、200 mg/kg)不仅能减轻大鼠肝纤维化程度,还能显著抑制IL-1、IL-6、NF-κB、TNF-α、TGF-β的表达,升高IL-10的水平。结论:PNS对肝纤维化大鼠具有一定的保护作用,其机制可能与下调促炎因子,上调抑炎因子的产生,调控细胞因子网络平衡有关。

Objective: To observe the effect of Panax notoginseng saponins( PNS) on cytokines production in liver fibrosis rats and its mechanism. Methods: SD rats were randomly divided into 6 groups,including normal group,liver fibrosis model group,PNS( 50,100,200 mg / kg) treated groups and Col group. Rat liver fibrosis was induced by CCl4twice a week for 18 weeks. PNS were administered daily from the 9th week for 10 weeks. The IL-1,IL-6,IL-10,TNF-α level in serum were determined by radioimmunity. The degree of liver fibrosis was observed by HE and immuninochemistry. The expression of TGF-β,TNF-α mRNA were detected by RT-PCR technology. Results: Compared with the fibrotic model group,PNS( 100,200 mg / kg) could not only significantly reduce histopathological change,but also effectively decrease the level of IL-1,IL-6,NF-κB,TNF-α,TGF-β,and increase the level of IL-10. Conclusion: PNS has protective effect on liver fibrosis and it maybe related to regulating cytokine network.