甜荞不同器官水提液体外抗氧化活性

Antioxidant Activities of the Aqueous Extraction from Various Organs of F. esculentum in Vitro

用NBT光化还原法、Fenton反应法和钼酸铵显色分光光度法测定了甜荞(F.esculentum Moench)不同器官(根、茎、叶和花)水提液体外抗羟基自由基(·OH)、超氧阴离子自由基(·O2-)和过氧化氢(H2O2)的能力。结果表明:甜荞不同器官水提液体外均能抗·OH、·O2-和H2O2。抗·OH的能力排序为:茎、花>叶、根;抗·O2-的能力排序为:花>叶、茎>根;抗H2O2的能力排序为:叶、花>根、茎。

The capacity of resisting ·OH, ·O2 and H2O2 of aqueous extractions from four organs （root,aerial stem,leaf and flower） of F. esculentum in vitro were estimated by means of nitro blue terra-zolium photochemica reduction,Fenton reaction and spectrophotometric assay. The results showed that the aqueous extractions had the capacity of resisting ·OH, ·O2- and H202. The capacity of resisting ·OH was sorted as ： aerial stem,flower 〉leaf,root. The capacity of resisting ·O2- was sorted as：flower〉leaf,aerial stem〉root. The capacity of resisting H2O2 was sorted as：leaf,flower〉 root, aerial stem.