液相色谱法定量检测老年人血浆游离脂肪酸

Quantification of Fasting Plasma Free Fatty Acids in Old Population by High Performance Liquid Chromatography

目的:分析老年人群空腹血浆游离脂肪酸水平及构成特点。方法:采用高效液相色谱法(high performance liquid chromatography,HPLC),检测149名60-104岁的健康老年人空腹血浆游离脂肪酸(Free Fatty acids,FFAs)的水平,包括月桂酸(C12:0)、二十碳五烯酸(C20:5)、豆蔻酸(C14:0)、软油酸(C16:1)、花生四烯酸(C20:4)、亚油酸(C18:2)、软脂酸(C16:0)、油酸(C18:1)和硬脂酸(C18:0)。结果:血浆FFA水平呈偏态分布;含量占血浆总FFA 10%以上的单个FFA有3种,分别为:C16:0,C18:1和C18:2,共占总FFA的80%。结论:C16:0,C18:1和C18:2为血浆总FFA的主要组分,可体现血浆总FFA水平,并给出了健康老年人群的血浆总FFA及9种亚组分的均值、四分位数及中位数水平。

Objective： To investigate the distribution characteristics and the levels of fasting plasma FFAs for healthy old popula- tions. Methods： High performance liquid chromatography （HPLC） was used to determine fasting plasma levels of C 12：0, C 14：0, C 16：0, C16：1, C18：0, C18：1 C18：2, C20：4 and C20：5 in 149 healthy individuals aged 60-104 years old. Results： The levels of fasting plasma FFAs are skewed distribution. The top three abundant FFAs, C16：0, C18：1 and C18：2 account for 80% of plasma total FFA, and each of them accounts for 10% of plasma total FFA. Conclusion： The major components of plasma FFA are C16：0, C18：l and C18：2, which could represent the level of plasma total FFA. Meanwhile, the average levels and percentiles of plasma total FFA and nine specific FFA are provided for healthy old populations.