柴胡疏肝散对肠易激综合征大鼠血清IL-6、TNF-α和5-HT的影响

Effect of Chai Hu Shu Gan decoction on serum IL-6、TNF-α and 5-HT in the rats model of irritable bowel syndrome

目的:观察柴胡疏肝散对肠易激综合征大鼠血清白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、5-羟色胺(5-hydroxytryptamine,5-HT)含量的影响,探讨该方治疗肠易激综合征的作用机制。方法:将60只健康清洁级Wistar大鼠,根据完全随机分组法分为空白组(A组)、模型组(B组)、对照组(C组)、高剂量组(D组)、中剂量组(E组)、低剂量组(F组),每组10只。除A组外,其余5组采用乙酸灌肠法造模。造模成功后,A组正常喂养,B组予生理盐水灌胃,其他组予相关药液灌胃,7天后观察大鼠一般情况并于3周后收集血标本,对IL-6、TNF-α、5-HT指标进行检测。结果:与B组比较,C组、D组、E组、F组中IL-6、TNF-α、5-HT含量下降(所有P<0.05)。D组IL-6、TNF-α、5-HT含量下降优于E组、F组及C组,差异均有统计学意义(所有P<0.05)。结论:柴胡疏肝散能够改善肠易激大鼠症状,并可能通过下调IL-6、TNF-α、5-HT的含量进而达到治疗目的。

objective：Observing the effect of Chai Hu Shu Gan San （CHSGS） on serum IL-6.TNF- α and 5-HT in the rats model of irritable bowel syndrome（IBS） to explore its theraptic mechanism on IBS.Methods：60 rats are divided into 6 groups：the blank control group（Group A）,the model group （ Group B ）,the control group （ Group C ）,the high dose of CHSGS group （ Group D）, the middle dose of CHSGS group （ Group E ）, the low dose of CHSGS group （Group F ）,there are 10 rats in each group.Except for the group A,rats from other 5 groups are treated with acetic acid by enteroclysm.After 1 week,rats from group A are fed normaly, rats from the model group are treated with physiological saline by gavage,rats from other groups are treated with different dose of CHSGS by gavarage.We observed the general state of health of rats after 7 days and detect their serum IL-6.TNF- α .5-HT after 3 weeks.Result： the level of serum IL-6.TNF- ct .5-HT in group C, group D,group E and group F are lower than group B（P 〈 0.05）. the level of serum IL-6.TNF- ct .5-HT in group D are lower than group E.group F and group C ,difference are stastically significant. （P 〈 0.05）.Conclusion ： CHSGS can ameliorate the symptom of the rats model of irritable bowel syndrome by lowering the level of serum IL-6.TNF-α .5-HT