老年人群Rb基因VNTR的多态性分布

Polymorphism of Rb gene VNTR in the aged people

目的 探讨老年人群 Rb基因 2 0内含子的 VNTR多态性分布 ,并与青年群体比较是否发生改变 ,以此研究老年人的衰老机制 .方法 对 5 6例陕西青年、6 8例老年人群用PCR- VNTR方法进行 Rb基因 2 0内含子多态性的检测 .结果 在青年群体发现 :等位基因片段 10个 ,片段大小为330～ 2 6 0 bp,重复片段大小为 5 bp,频率分布在 0 .0 0 9～0 .2 95 .该位点杂合率为 :0 .70 ,PIC(信息量 ) :0 .86 .老年人群多态片段增多至 18个等位基因片段 ,范围在 2 40～ 390 bp,青年人群及老年人群的 Rb基因 VNTR位点的多态分布差异有显著性 (P<0 .0 0 1) ,同时发现 VNTR重复数量 :老年人群 >青年人群 .结论 Rb基因

AIM To investigate distribution of polymorphism of Rb gene intron 20 VNTR in the aged and whether there is change between youth and the aged in polymorphism of Rb gene intron20 VNTR, To study whether polymorphism of Rb gene VNTR is associated with cellular aging. METHODSPolymorphism of Rb gene (intron 20) VNTR was examined in 56 cases of unrelated youth and 68 aged people by polymerase chain reaction (PCR). RESULTS There were 10 alleles ranging from 260 bp to 330 bp in youth. Repeat base was 5. The allele frequencies were 0.0009~0.295. The heterozygosity of Rb (intron 20) locus was 0.70. Polymorphism information content (PIC) was 0.86. There were 10 alleles ranging from 240 bp to 390 bp in youth. Distribution of genetic polymorphism of Rb gene (intron 20) VNTR was significantly different between youth and the aged (P<0.001), CONCLUSION Change of Polymorphism of Rb gene VNTR may be one of the mechanisms of cellular aging.