光镜下伯氏嗜木螨主要发育期的形态学观察

Morphological observation of Calogyphus berlesei at its major development stages

目的:观察伯氏嗜木螨的主要发育期形态。方法:采集某饲料厂饲料样本,分离其中孳生螨,纯化后清洗,制备标本玻片,光学显微镜下观察鉴定。结果:光镜下见雌性成螨躯体无色,表皮光滑有光泽,足4对呈淡棕色,体圆且膨胀明显,背部刚毛4对。除顶内毛外,所有躯体背面刚毛几乎完全光滑并在基部加粗,生殖感觉器大且明显,肛门周围6对微小的肛毛。雄性成螨螨体较雌螨小,呈纺锤状,背面刚毛较雌螨长,除生殖区有明显骨化的阳茎和圆形的肛门吸盘外,其他结构与雌螨基本相同。卵长椭圆形,无色半透明,内常见未发育成熟的幼螨。幼螨足3对,在基节Ⅰ区可见较长的中空管子———基节杆(CR)。结论:光镜下观察到伯氏嗜木螨的主要发育期形态,拟为鉴定此螨提供更加直观与客观的依据。

Objective：To observe the external morphology of Calogyphus berlesei（C.berlesei） in its major development stage.Methods：The feed samples were obtained from a feed mill,and the mites were isolated and purified via rinsing.After preparation of the glass samples,the specimens were observed with light microscope.Results：Under the light microscope, the adult female mites exhibits colorless idiosoma ,with smooth and bright external surface and 4 pairs of light brownish legs.The idiosoma looks much more rounded and expanded, and 4 pairs of setae arise from the back.The entire bristles at the dorsal area appear totally smooth and wid-en at their bases except internal verticals（vi）.The genital sensillum is larger and apparent,and 6 pairs of anal setae are slim,arising from around the anus.Contrarily,the idiosoma of an adult male is smaller than the fe-male,and exhibits itself with spindly shape which has longer setae .The remaining structures look similar to that of the female′s except for typical-ly ossified penis and round anal sucker seen at the genital area.The egg, in which immature larvae are more frequent,is ovally shaped,offwhite and semitransparent.The larva bears 3 pairs of legs,and the coxal rod（CR）is well-developed,which is in nature a hollow canal extended from the tip head .C onclusion：The morphology for C.berlesei examined at its major development stage will supply direct and objective evidence for identifica-tion of this mite .