哈尔滨地区猪源多重耐药大肠杆菌接合性质粒和整合子检测

Inspection of multidrug resistant Escherichia coli conjuagal plasmid and derived from swine in Harbin

为调查哈尔滨周边地区猪源多重耐药大肠杆菌流行情况,检测与水平转移密切相关接合性质粒traA、trbC及Ⅰ型、Ⅱ型、Ⅲ型整合子分布状况。对哈尔滨周边地区发病猪场采样处理,得到24株大肠杆菌。采用Kirby-Bauer法对24株大肠杆菌进行14种药物药敏试验,并采用聚合酶链式反应(PCR)法分析接合性质粒遗传标记traA、trbC及Ⅰ型、Ⅱ型、Ⅲ型整合子。结果显示大多数猪源大肠杆菌对14种抗菌药物均表现出较高耐药性,有些细菌甚至对其中12种药物产生耐药性。试验细菌对氟苯尼考、磺胺甲恶唑/甲氧苄啶(SXT)和多西环素(DOX)耐药率最高,分别可达100%、95.83%和83.33%。traA和trbC检出率分别为91.67%(22/24)、62.5%(15/24),Ⅰ型、Ⅱ型、Ⅲ型整合子检出率分别为91.67(22/24)、12.5(3/24)和0。结果表明哈尔滨周边地区猪源大肠杆菌耐药现象十分严重,接合性质粒和整合子检出提示可能存在耐药性水平转移现象。

The purpose of this paper is to investigate the multidrug resistance of swine Escherichia coli in Harbin, detecting the distribution of conjuagal plasmid traA, trbC and the I, II, III integron which is closely related to the transfer of resistance in level. In this study, 24 strains of E.coli had been sampled and isolated from invasive swines in Harbin. The resistance phenotypes to 14 kinds of antimicrobial agents were tested by Kirby-Bauer method, and the genetic markers of conjuagal plasmid traA, trbC and the I, II, III integron was analysised by polymerase chain reaction （PCR）. The results showed that most of the E..coli isolated from swine showed higher resistance to 14 kinds of antimicrobial agents, and even some bacteria resistance to 12 kinds of antimicrobial. The testing bacteria had highest drug resistance rate to Florfonicel,Sulfamethoxazole/Tdmethoprim （SXT） and Doxycycline （DOX）, the rate were 100%, 95.83%, 83.33%, respectively.The positive rate of traA, trbC and the I, II, III integron is 91.67%（22/24）, 62.5%（15/24） and 91.67（22/24）, 12.5（3/24）, 01 respectively. Results indicated the antimicrobial resistance is very serious in Harbin, there may be exist of the phenomenon of the resistance transfer in level.