太湖鹅与皖西白鹅IGF-I和MSTN mRNA表达与屠宰性状相关性分析

Gene expression profile of IGF-I, MSTN mRNA and their correlation with carcass traits in Taihu goose and Wanxi goose(Carassius auratus Gibelio) using UPLC/Q- TOF MS

文章以太湖鹅和皖西白鹅为研究素材,采用多重荧光竞争性PCR方法研究鹅70日龄胸肌和腿肌中IGF-I、MSTN mRNA表达情况,并与屠宰性能做相关性分析,探讨IGF-I、MSTN对鹅骨骼肌生长的影响。结果表明,胸肌IGF-I mRNA表达量太湖鹅极显著高于皖西白鹅,腿肌MSTN mRNA表达量太湖鹅显著高于皖西白鹅,胸肌MSTN mRNA、腿肌IGF-I mRNA没有品种差异性。鹅群体,腿肌IGF-I mRNA表达量公鹅极显著大于母鹅,MSTN mRNA表达量腿肌极显著高于胸肌,IGF-I mRNA表达量没有组织差异性。胸肌和腿肌组织中IGF-I mRNA表达量和MSTN mRNA表达量极显著正相关,腿肌IGF-I mRNA表达量和胸肌率显著负相关,皖西白鹅腿肌MSTN mRNA表达量与体重、腿肌重显著负相关。

In this study, the expression of IGF-I and MSTN mRNA in breast and leg muscle was analyzed in 70 days old Taihu goose and Wanxi goose with Multiplex Competitive Fluorescent-PCR method, and the correlations between mRNA levels and carcass traits were analyzed to study the effects of mRNA levels on muscle growth. The result showed that IGF-I mRNA level of breast muscle in Taihu goose was significantly higher than that in Wanxi goose, MSTN mRNA level of leg muscle in Taihu goose was significantly higher than that in Wanxi goose. There was no significant difference in breast muscle MSTNlevel and leg muscle IGF-I mRNA level between the two breeds. In the same breed, IGF-I mRNA level of leg muscle in male goose was significantly higher than that in female goose, and MSTN mRNA level of leg muscle was significantly higher than that in breast muscle. There was no difference in IGF-I mRNA level between viable tissues. There was positive correlation between IGF-I mRNA and MSTN mRNA level both in breast muscle and leg muscle, and there was negative correlation between IGF-I mRNA level of breast muscle and leg muscle rate. In Wanxi goose, MSTN mRNA level of leg muscle was negatively associated with body weight and leg muscle weight.