酶法拆分DL-β-乙酰硫基α-甲基丙酸

Study on the Enzymic Resolution of DL-β-Acetylthio-α- Methylpropionic acid

研究了胰酶和脂肪酶对DL 加成酸的拆分效果和各种影响因素 ,结果表明 ,脂肪酶Ⅰ的拆分效果优于胰酶和脂肪酶Ⅱ。脂肪酶Ⅰ酶促反应的最优条件为 :底物 4.0 g ,酶用量 1.5 g ,反应时间 12h ,反应体系pH7.0。拆分结果得到D 加成酸。

Pancreatin, lipaseⅠandⅡ were used to resolute DL -β-acetylthio-α-methylpropionic acid DL -AT to obtain D -AT. The result showed that the lipase Ⅰ had better resolution result than pancreatin and lipaseⅡ. A number of factors have effects on the resolution result of lipaseⅠ.The optium resolution condition was observed by using DL -AT 4.0 g, lipase Ⅰ1.5 g,the pH of reaction system 7.0,reaction time 12 h.