针刺对哮喘患者外周血单个核细胞培养上清中IL-4分泌活性、血清总IgE水平的影响

Effect of Acupuncture on IL-4 Activity in Cultured PBMC and the Serum IgE Level in Bronchial Asthma Patients

采用针刺治疗过敏性哮喘 2 5例 ,其中轻度 7例 ,中度 1 8例 ,治疗 1个疗程后观察疗效及治疗前后患者外周单个核细胞 (PBMC)培养上清中IL 4分泌活性、血清总IgE的变化情况。结果表明 ,临床疗效显著 ,总有效率达 96 %。治疗前患者PBMC培养上清中IL 4分泌活性、血清总IgE水平均显著地高于健康对照组 (P <0 .0 1 ) ,治疗后 ,患者上述指标均有明显下降 ,与疗前比较有显著性差异 (P <0 .0 5 )。提示针刺能抑制过敏性哮喘患者PBMC培养上清中的IL 4分泌活性 ,降低血清总IgE的水平 ,从而阻断IgE介导的Ⅰ型超敏反应 ,这可能是针刺治疗过敏性哮喘取效的主要机制之一。

cases of allergic bronchial asthma were treated by acupuncture of Dazhui (GV 14), Feishu (BL 13),Dingchuan (EX B 1), Pishu (BL 20), Shenshu (BL 23), Fengchi (GB 20), etc. Among them, 7 cases were mild type and 18 moderate type. Supernate IL 4 activity in cultured PBMC (peripheral B mononuclear cell) derived from asthma patients was determined by using enzyme linked immunosorbent assay and serum total IgE content determined by using double antibody sandwich enzyme linked immunosorbent assay. After one course of treatment, results revealed that patients' symptoms were improved remarkably, with the total effective rate being 96%. Before treatment, supernate IL 4 activity in cultured PBMC and the total content of IgE in serum were significantly higher than those of healthy subjects(25 cases, P<0.01)). After treatment, the above mentioned index values lowered obviously in comparison with pre treatment (P<0.05). The fact suggests that acupuncture can inhibit IL 4 activity and lower serum total IgE content in allergic bronchial asthma patients.