雷公藤叶片总RNA提取方法初探

Study on the RNA extraction method of Tripterygum wilfordii leaves

以3年生雷公藤实生苗幼嫩叶片及雷公藤组培苗为提取材料,采用CTAB、CTAB-LiCl、CTAB-Trizol、Trizol、天根试剂盒以及百泰克试剂盒等6种方法,提取雷公藤叶片总RNA。结果表明,CTAB法及CTAB-LiCl法经纯化、浓缩后得到的RNA完整性较好且纯度较高,可以用于后续研究。

Triennial seedling and tissue culture seedling of Tripterygum wilfordii were used to extract total RNA with CTAB, CTAB- LiCI, CTAB-Trizol, Trizol, TIANGEN RNAprep Pure Plant Kit and Bio Teke RNApure Total RNA Plant kit method. It was showed that CTAB and CTAB-LiCI method provided more integrated total RNA than any others, and could be used for further research.