人参茎叶皂甙和胆碱合用对喹啉酸损毁基底核大鼠学习记忆的影响

Effects of ginsenoside of stem and leaf in combination with choline on learning and memory in quinolinic acid induced lesion in nucleus basalis of Meynert of rats

应用喹啉酸 (每侧 1 50 nmol,2 μL,给受试药后 d3注射 )损毁老年大鼠双侧 Meynert基底核制备阿尔茨海默病 (AD)动物模型 ,放射化学法测定胆碱乙酰基转移酶 (Ch AT)活性 ,一次性训练被动回避跳台实验和水迷宫空间分辨能力测试 ,观察人参茎叶皂甙 (GSL )和胆碱合用对 AD模型大鼠学习记忆的影响 .结果显示 :GSL(40 0 mg·kg-1·d-1)和胆碱 (2 0 0 mg·kg-1· d-1)合用 ig给药 ,大鼠在跳台中出现的错误反应次数 (给药 1 3d)和学会迷宫训练次数 (给药 1 6d)显著减少 ,大脑皮层 Ch AT活性明显升高 (给药 1 9d) ,且均比两药单用效应明显 ,呈协同作用 .表明 GSL和胆碱合用有协同改善 AD模型大鼠学习记忆障碍的作用 ,其机理与协同提高Ch AT活性 。

Alzheimer′s disease (AD) animal models were made by damaging bilateral nucleus basalis of Meynert (NBM) of elderly rats (20-22 month) with quinolinic acid(150 nmol in 2 μL for each NBM, given d 3 after administration of test drugs). Activity of choline acetyltransferase(ChAT) was measured by radiochemistry assay. One time training passive avoidance step down and water maze spatial localization task were used to observe effects of ginsenoside of stem and leaf(GSL) in combination with choline on learning and memory of AD model rats. The result showed that by oral administration of GSL(400 mg·kg -1 ·d -1 ) in combination with choline(200 mg·kg -1 ·d -1 ), the number of error in step down test after 13 d administration and the training times to reach the criterion on water maze task after 16 d administration were decreased significantly. Whereas activity of ChAT was increased obviously after 19 d administration. The effect of GSL in combination with choline was more remarkable than that of GSL or choline alone which suggests synergic effect. So GSL in combination with choline can synergically reduce the impairment of learning and memory of AD model rats and the mechanism may be involved in enhancing the function of central cholinergic system.