自发性2型糖尿病模型db/db小鼠生物学特性研究

Study on biological characteristics of spontaneous type 2 diabetic model db/db mice

目的对db/db小鼠进行生物学特性研究,满足糖尿病研究的需要。方法在试验周期内,测定db/db小鼠的空腹血糖、体质量及摄食饮水量,并测定21周龄模型动物的糖耐量、血中胰岛素、胰高血糖素、三酰甘油、总胆固醇等指标的水平,进行胰腺的免疫组化双重染色及主要脏器的病理学检查,并与db/m小鼠进行比较。结果与对照组db/m小鼠比较,db/db小鼠过度肥胖,伴有高血糖、胰岛素抵抗、脂质代谢紊乱,且肝脏和胰腺组织均出现明显病变,胰腺免疫组化双重染色结果与血液学测定结果一致,并可观察到胰岛中A细胞和B细胞的分布变化情况。结论对自发性2型糖尿病模型db/db小鼠所做的相关生物学特性检测,可供糖尿病研究参考。

Objective To meet the need of study on diabetes mellitus,we investigated the biological characteristics of db/db mice.Methods In this experiment,fasting blood glucose,body weight,food intake,and water intake were measured.Fasting blood glucose levels in oral glucose tolerance test were observed on db/db mice of 21 weeks old.Meanwhile,the mice were sacrificed for the detection of insulin,glucagon,triglyceride（TG）,and total clolesterol（TC） in serum,immunohistochemical double staining in pancreas,and morphological study.All the data of db/db mice were compared with those of db/m mice in statistics.Results Compared with db/m mice,obesity,hyperinsulinemia,hyperglycemia,and lipid metabolism disorder were serious on db/db mice,following obvious pathological deterioration of liver and pancreas.In accordance with the result of blood testing,change in distribution of A cells and B cells could be observed in immunohistochemical double staining on pancreas of db/db mice.Conclusion The db/db mice could be used as appropriate disease models of type 2 diabetes mellitus,providing the relevant biological information for further study in this field.