PAⅠ-1蛋白水平在口腔颌面部肿瘤中的研究

PAI-1 protein level in oral and maxillofacial tumors

目的 :了解口腔颌面部肿瘤组织中 型纤溶酶原激活剂抑制剂 (palsminogen activator inhibitor- 1,PA - 1)蛋白表达情况 ,分析其与各病理参数间的关系。方法 :采用发色底物法、EL ISA夹心法测定 30例口腔颌面部恶性肿瘤组织、相应瘤旁组织及 10例良性肿瘤组织的 PA - 1活性及含量。结果 :恶性肿瘤组织 PA - 1活性及含量明显高于良性肿瘤及瘤旁组织 (P<0 .0 1) ;PA - 1活性及含量与临床分期相关(P<0 .0 1和 P<0 .0 1) ,与颈淋巴结转移明显相关 (P<0 .0 1)。结论 :PA - 1在口腔颌面部恶性肿瘤中有高表达 ,且与肿瘤的侵袭和转移有关 ,在蛋白水平上检测 PA - 1对判断有无转移、指导手术、评估预后有帮助作用。

砄bjective:To investigate the expression of plasminogen activator inhibitor 1(PAI 1) in oral and maxillofacial tumors and the relationship between PAI 1 and pathological parameters.Methods:Chromogenic substrate assay was used to determine PAI 1 level in tumor tissues and ELISA was used to detect the concentration of PAI 1 in tissue extracts in 30 cases of malignant tumors and 10 of benign tumors in oral and maxillofacial area.Results:Higher level and concentration of PAI 1 were found in malignant tumors than in tumor adjacent tissues or benign tumors ( P <0.01).Higher PAI 1 level and concentration were also observed in the later stages of the tumors than in earlier ones ( P <0.01 and P <0.05 respectively) and in the cases with lymph metastasis than in those without metastasis ( P <0.01).Conclusion:PAI 1 is highly expressed in oral and maxillofacial malignant tumors,and PAI 1 concentration is positively correlated to trmor invasion and metastasis.