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黄肉甘薯糖蛋白提取工艺的研究 被引量:2

Study on the Extraction Technology of Glycoprotein from Yellow Flesh Sweet Potato
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摘要 以黄肉甘薯为材料,探索了水提法制备甘薯糖蛋白的工艺。通过单因素和正交试验,得出制备甘薯糖蛋白的最优工艺条件:物料粒度为80目,浸提时间为1h,浸提温度为50℃,料液比为1∶15,浸提次数为1次。在该优化条件下甘薯糖蛋白的含量为17.730mg/g。方差分析表明,物料粒度、浸提时间、浸提温度和料液比对甘薯糖蛋白提取率的影响均不显著。 We studied the technology for extracting glycoprotein from yellow flesh sweet potato by water extraction. Based on the single factor and orthogonal experiments, we determined the optical extraction conditions: material granularity was about 80 meshes,extraction time 1.0 hour,extraction temperature 50℃,solid to liquid ratio 1:15, and extraction once.Under the optimal conditions, the glycoprotein from sweet potato was up to 17.730mg/g.The variance analysis showed that material granularity, extraction time, extraction temperature and solid to liquid ratio had no significant effect on the extraction rate.
出处 《安徽农学通报》 2013年第19期119-121,共3页 Anhui Agricultural Science Bulletin
基金 河南工业大学博士基金项目(2010BS020) 郑州市科技攻关项目(20110314)
关键词 甘薯 糖蛋白 提取 单因素 正交试验 Sweet potato Glycoprotein Extraction Single factor Orthogonal test
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参考文献4

  • 1Geyer H, Geyer R.Strategies for analysis of glycoprotein glycosyl- ation [J].Biochim Biophys Acta,2006,1764(12) : 1 853-1 869.
  • 2吕克凡,高世勇.糖蛋白抗肿瘤研究进展[J].齐齐哈尔医学院学报,2012,33(12):1642-1643. 被引量:4
  • 3程坷伟,许时婴,王璋.甘薯糖蛋白的分离纯化和组成[J].食品与发酵工业,2004,30(11):130-134. 被引量:15
  • 4Kusano S.Isolation of antidiabetie comonents from white-skinned sweet potato (Ipomoea batatas L)[J].Biosci Biotedhnol Biochem, 2001,65(1) : 109-114.

二级参考文献21

  • 1祝雯,林志铿,吴祖建,林奇英,谢联辉.河蚬中活性蛋白CFp-a的分离纯化及其活性[J].中国水产科学,2004,11(4):349-353. 被引量:20
  • 2杨立明,陈赐民.浅谈甘薯综合开发利用[J].国外农学(杂粮作物),1995(2):44-45. 被引量:61
  • 3韩雅莉,谢昆.土鳖虫糖蛋白的提取及抗肿瘤活性初步研究[J].汕头大学学报(自然科学版),2006,21(4):46-50. 被引量:18
  • 4刘主,朱必凤,彭凌,高建林.甘薯糖蛋白SPG-1抗肿瘤及免疫调节作用研究[J].食品科学,2007,28(5):312-316. 被引量:15
  • 5张维杰.糖复合物生化研究技术[M].浙江大学出版社,1994,10.16-17.
  • 6Satoshi Kishino, Katsumi Miyazaki. Separation methods for glycoprotein analysis and preparation[J]. Journal of chromatography B, 1997,699:371~381
  • 7Othmar Gabriel, Douglas M. Spaining for enzymatic activity after gel electrophoresis [J]. Gersten Anal Biochem, 1992(203): 1~21
  • 8Lowry,O .H et al, Protein measurement with the folin phenol reagent[J]. Biol Chem, 1951, 193(1):265~267,1951
  • 9Englyst H, Wiggins H S Cummings J H, Determination of the non-starch polysaccharides in plant foods by Gas-Liquid chromatography of constituent sugars as alditol acetates [J]. Analyst, 1982,107:307~318
  • 10Albert S B Edge, Robert G Spiro. Structure of the O-Linked oligosaccharides from a major Thyroid cell surface glycoprotein [J]. Archives of Biochemistry and Biophysics, 1997,343 (1): 73~80

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