四君子汤对脾气虚证模型大鼠小肠葡萄糖吸收功能作用的影响

Effect of Sijunzi Decoction on the Intestinal Glucose Absorption in Model Rats of Pi-qi Deficiency Syndrome: an Experimental Research

目的观察四君子汤对脾气虚证模型大鼠小肠葡萄糖吸收功能的影响。方法通过颈部皮下注射利血平建立脾气虚证大鼠模型,观察大鼠体重及尿D-木糖排泄率变化,通过在体空肠灌流收集并检测葡萄糖吸收量,收集小肠黏膜,检测葡萄糖运载蛋白2(GLUT2)蛋白及mRNA表达变化。结果与正常对照组比较,模型组大鼠体重及尿D-木糖排泄率均降低,同时伴有葡萄糖吸收的下降以及GLUT2蛋白及mRNA表达的降低,通过给予四君子汤治疗,四君子汤组在各指标恢复上均优于自然恢复组。结论四君子汤能够促进利血平致脾气虚证模型大鼠小肠吸收葡萄糖功能恢复。

Objective To observe the effect of Sijunzi Decoction （SD） on the intestinal absorption of glucose in model rats of Pi-qi deficiency syndrome （PQDS）. Methods PQDS rat model was established by subcutaneous injection of reserpine from the neck. The body weight and urine D-xylose excretion rates were measured. The glucose uptake rate was measured by jejunum perfusion. The intestinal mucosa was collected. The glucose transporter-2 （GLUT2） protein and mRNA expression levels were detected. Results Compared with the normal control group, the body weight and D-xylose excretion rates decreased in the model group. Meanwhile, the glucose uptake and GLUT2 protein and mRNA expression levels decreased in the model group. The aforesaid indices were improved in the SD group. Conclusion SD could promote the recovery of glucose uptake in the small intestine of reserpine induced PQDS rats.