枸杞多糖对糖尿病大鼠视网膜超微结构的影响

Effect of Lycium Barbarum Polysaccharides on the Retinal Ultrastructure of Streptozocin-induced Diabetic Rats

目的研究早期糖尿病大鼠视网膜超微结构的改变及枸杞多糖的干预效果。方法链脲佐菌素(STZ)诱导糖尿病SD大鼠模型,治疗组给予枸杞多糖灌胃,观察血糖、体重变化和24周时的视网膜超微结构改变。结果糖尿病早期视网膜改变主要是神经元细胞和神经胶质细胞等神经组织的线粒体病变和细胞的变性、凋亡。枸杞多糖组与糖尿病模型组比较,体重和血糖差异均无统计学意义(P>0.05);而视网膜超微结构病变明显减轻,且局限于内核层。结论枸杞多糖可以明显减轻线粒体的病理改变,阻止神经细胞凋亡,其机制并非通过降低血糖,控制糖尿病发挥作用,有望用于早期糖尿病视网膜神经病变的防治。

Objective To study the retinal ultrastructure of streptozocin （STZ）-induced diabetic rats and the intervention effect of Lycium Barbarum Polysaccharides （LBP）. Methods The STZ-induced diabetic SD rat model was established. LBP was given to those in the treatment group by gastrogavage. Changes of body weight, blood glucose, and retinal ultrastructure at 24-week were observed. Results Early retinal changes covered mitochondrion changes, cell degeneration and apoptosis of retinal neurons and neuroglia cells in the diabetic rats. No change of body weight or blood glucose was observed between the LBP group and the diabetic model group （P 〉0.05）. The ultrastructural changes were obviously re- lieved by LBP, and limited to the in＇her nuclear layer. Conclusions LBP could obviously relieve patholog- ical changes of mitochondrion, hinder neural cell apoptosis. Its effect might not be achieved by lowering blood glucose. It was expected to be used in preventing and treating early diabetic retinal neuropathy.