游泳运动后自发性高血压大鼠主动脉一氧化氮的变化及其对胱硫醚-γ-裂解酶/硫化氧体系的影响

Effects of swimming exercise on nitric oxide and CSE/H_2S in spontaneously hypertensive rats

目的:观察运动对自发性高血压大鼠(SHR)主动脉一氧化氮合酶(NOS)、一氧化氮(NO)、胱硫醚-γ-裂解酶(CSE)和硫化氢(H_2S)的影响,探讨运动干预后SHR大鼠内源性NO的变化对主动脉CSE/H_2S体系的调节作用。方法:选用雄性SHR大鼠16只,随机分为高血压对照组(SC组)和高血压运动组(ST组),每组8只。同时选用雄性Wistar大鼠8只,为正常对照组(WC组)。ST组大鼠进行8周、每周6次、每次90min中等强度的无负重游泳运动。结果:8周90min游泳运动后,SC组大鼠血压较实验前显著升高(P<0.01),ST组大鼠血压较SC组大鼠血压显著下降(P<0.01),且与实验前相比无显著性差异(P>0.05);ST组大鼠较SC组大鼠主动脉NOS、NO、CSE和H_2S水平显著升高(P<0.05)。结论:运动可抑制SHR的血压上升,增加SHR主动脉NOS和NO的含量,内源性NO对SHR主动脉CSE/H_2S体系在运动缓解血压上升中呈促进作用,这一过程参与了运动降压的调控机制。

Objective： To study the effects of exercises on blood pressure and aorta nitric oxide synthase（NOS）,nitric oxide （NO）, cystathionine-7-1yase（CSE） and hydrogen sulfide in spontaneously hypertensive rats （SHR）, then to ex- plore the regulation effects of exercise on endogenous NO and aorta CSE/H2S in SHR. Method： Sixteen male SHR were randomly divided into the SHR control group（SC） and SHR training group （ST）; 8 rats in each group. Eight male Wistar rats were the normal matched control group（WC）.ST group car- ries on 90 min moderate swimming, without load, 6 times a week. All rats feed normal feedstuff for 8 weeks. And then the rats＇ blood pressure（BP） were measured each week during experimental period, the contents of aorta NOS,NO, CSE and H：S were examined. Result： After 8-weeks swimming exercise, the BP in SHR training group was little higher than before, but no significant difference. However, in SHR control group it was just opposite （P〈0.01）; the levels of aorta NOS, NO, CSE and H2S in SHR training group were significantly elevated（P〈0.05）. Conclusion： Swimming exercise can inhibit the elevation of BP, increase the levels of aorta NOS and NO in SHR, and endogenous NO can promote the aorta CSE/H^S system in the process of exercise depressing the blood pressure elevation.