女贞子提取物主要活性组分及其抗丙型肝炎病毒复制活性分析

Main Active Components of Ligustrum lucidum Extract and Their Activity to Inhibit Hepatitis C Virus Replication

中药女贞子(Ligustrum lucidum,LL)具有肝保护和抗炎症作用.本研究分析女贞子提取物对丙型肝炎病毒(hepatitis C virus,HCV)复制的影响及其活性成分.薄层层析法分离女贞子水提取物,获得5个分离组分.Real-time RT-PCR和Western印迹发现,分离组分1和2抑制HCV JFH1细胞感染模型中的JFH1病毒复制.分离组分的高效液相色谱(high-performance liquid chromatography,HPLC)分析表明,熊果酸和齐墩果酸可能是组分1和2的抗病毒活性成分.熊果酸和齐墩果酸抗病毒实验发现,熊果酸和齐墩果酸抑制HCV JFH1的复制,它们的选择指数(SI)分别为6.7和30.8.这些研究结果表明,女贞子及其化学成分熊果酸和齐墩果酸具有潜在的丙型肝炎治疗价值.

The Chinese herb Ligustrum lucidum （LL） exhibits hepatoprotective and anti-inflammatory activities. In this study, we investigated the effect of LL extract on hepatitis C virus （HCV） replication and its active compound （s）. The aqueous LL extract was separated to five fractions on thin layer chromatography （TLC） plate. Among these five fractions, only fractions 1 and 2 reduced HCV JFH1 replication in JFH1 cell infection model by real-time RT-PCR and Western blot analysis. High- performance liquid chromatography （HPLC） analysis showed that ursolic acid and oleanolic acid may be two antiviral components within fractions 1 and 2 which significantly suppressed the replication of HCV JFH1 virus. Direct antiviral assays using ursolic acid and oleanolic acid demonstrated that ursolic acid and oleanolic acid inhibited the replication of HCV JFH1 virus. The selectivity index （SI） values of ursolic acid and oleanolic acid against JFH1 virus replication are 6.7 and 30.8. These results indicated that LL extract and its active compounds including ursolic acid and oleanolic acid might offer a promising source of antiviral drugs against hepatitis C virus.