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Sirt3在不同热量饮食下的大鼠脂肪组织中的表达 被引量:2

Expression of Sirt3 in Adipose Tissue of Rats on Diets with Different Calories
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摘要 Sirt3是一种NAD+依赖性组蛋白去乙酰化酶,在肾脏、棕色脂肪、心脏和其它代谢活跃的组织中高表达.多项研究表明,Sirt3在细胞能量代谢、衰老、肿瘤发生等方面起着重要的作用.为探讨Sirt3基因在不同热量饮食下的SD大鼠脂肪组织中的表达差异和意义,将60只3周龄,重量(60±5)g的断乳SD雄性大鼠,随机分为热量限制组、自由摄食组、高热量组,其中高热量组用于构建肥胖SD大鼠模型,喂养16周后处死,分别用Western印迹和real-time RT-PCR方法检测各组大鼠肾周脂肪组织中Sirt3基因的蛋白和mRNA的表达量.Western印迹检测与real-time RT-PCR检测结果一致:Sirt3基因的蛋白和mRNA在肥胖大鼠脂肪组织中的表达低于自由摄食组(P<0.05),在热量限制组大鼠脂肪中的表达高于自由摄食组(P<0.05).研究结果说明,Sirt3基因在不同热量饮食下的SD大鼠脂肪组织中的表达有差异,可能与能量代谢及肥胖有着密切关系.
出处 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2013年第10期990-994,共5页 Chinese Journal of Biochemistry and Molecular Biology
基金 福建省自然科学基金(No.2012J01125) 福建医科大学教授学术发展基金(No.JS10011)资助~~
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