^(60)Coγ射线照射后培养小鼠骨髓基质细胞内NF-κB的变化

Changes of nuclear factor-κB (NF-κB) in cultured bone marrow stromal cells after ^(60)Coγ-irradiation \

目的 探讨辐射对培养小鼠骨髓基质细胞内NF κB的影响。方法 采用免疫组化、Westernblot及凝胶电泳迁移率实验。结果 免疫组化方法显示正常骨髓基质细胞细胞核的周围表达低水平的NF κB ,8 0Gy60 Coγ射线损伤后 1h即见其表达较正常显著升高并出现明显的核移位 ,4h达高峰 ,6h有所回落 ,8h恢复正常 ;Westernblot显示照射后骨髓基质细胞NF κB的蛋白表达量较正常明显升高 ,8h仍然未恢复正常 ;凝胶电泳迁移率实验发现正常骨髓基质细胞内存在低水平活性NF κB ,8 0Gy60 Coγ射线损伤后 1h即见其活性明显升高 ,4h达高峰 ,8h恢复正常。结论8 0Gyγ射 线损伤后培养小鼠骨髓基质细胞内NF κB的表达升高并激活 ,这可能与骨髓微环境内基质细胞的抗辐射能力及造血功能的恢复有关。

Objective\ To explore the changes in bone marrow hematopoietic microenvironment after exposure to γ-radiation. Methods \ Immunohistochemistry,Western blot and electrophoretic mobility shift assay (EMSA)was used. Results The nuclear translocation was found in cultured bone marrow stromal cells after 60 Coγ-irradiation with immunohistochemistry.The expression of NF- κB in the bone marrow stromal cells (BMSCs) on the level of protein was elevated after exposure to 60 Coγ-rays at the dose of 8 0Gy with the uses of immunohistochemistry and Western blot.The activities of NF-κB in the BMSCs increased significantly after exposure to γ-radiation by using EMSA.The activity peak was at 4 hours after irradiation. Conclusion \ Our results suggest that the activation of NF-κB in the BMSCs by irradiation might be involved in the protection of BMSCs and in the recovery of hematopoiesis after γ-irradiation.\;