胚胎期受辐射对子鼠造血基质细胞功能的影响

Effects of prenatal irradiation on the functions of murine haematopoietic stromal cells.\

目的 研究胚胎期 2 0Gy137Csγ射线照射后对子鼠造血基质细胞的远期影响。方法 建立胚胎期受辐射小鼠模型 ;以细胞计数检测股骨骨髓有核细胞 (BMNCs)数 ;用免疫组化ABC法检测基质细胞粒单系集落刺激因子 (GM CSF)和干细胞因子 (SCF)的表达 ;用体外小鼠骨髓细胞培养法以粒单细胞系祖细胞集落形成单位 (CFU GM )为指标观察基质细胞对正常骨髓造血的支持功能。结果 胚胎期受辐射小鼠BMNCs数明显低于正常小鼠 (P <0 0 1) ,其骨髓基质细胞生长因子GM CSF和SCF的表达均高于正常小鼠 (P <0 0 5 ) ,同时 ,胚胎期受辐射小鼠骨髓基质细胞对正常骨髓造血的支持能力也高于正常小鼠 (P <0 0 5 )。结论 2 0Gy137Csγ射线对发育中的小鼠胚胎的造血基质细胞产生了明显影响。

Objective\ To observe the effects of prenatal irradiation on functions of haematopoietic stromal cells. Methods \ Long-term effects of 2 0Gy 137 Cs γ-radiation at midterm gestation of mice on the haematopoietic stromal cells of their offspring were studied. Results \ The quantity of femoral bone marrow nucleated cells (BMNCs) in the irradiated offspring was markedly lower than that of the normal offspring ( P <0 01).And stromal expressions of the growth factors,granulocyte-macrophage colony-stimulating factor (GM-CSF) and stem cell factor (SCF),of the irradiated offspring were higher compared with those of the normal offspring ( P<0 05 ).At the same time,the ability to support heamatopoiesis by the stromal cells in irradiated offspring was higher than that in normal offspring. Conclusion \ The results suggest that irradiation with 2 0Gy 137 Cs γ-rays is sufficient to produce measurable effects on developing murine haematopoietic stromal cells.\;