异体神经干细胞山羊脑皮层定点移植后存活分化情况的实验研究

Survival and differentiation of extrinsic neural stem cells being transplanted into goats＇ brain cortices

目的研究异体神经干细胞大动物脑皮层内定点移植后的存活及分化情况。方法利用大动物（山羊）作为研究对象，通过立体定向方式将用绿色荧光蛋白标记过的异体神经干细胞定点移植到山羊受损脑组织周围皮层内。1个月后处死动物，取移植部位脑组织进行冰冻切片免疫组化染色检测，检测指标为神经系统的3种主要组成细胞（神经元、星形胶质细胞及少突胶质细胞）的特异性标记物——微管相关蛋白2（MAP21、神经纤维酸性蛋白（GFAP）及少突胶质细胞标志蛋白01（01）。结果移植1个月后，山羊脑皮层内的异体神经干细胞不仅能在宿主体内存活，还能分化为神经系统的3种主要组成细胞——神经元、星形胶质细胞及少突胶质细胞，其中表达MAP2及GFAP的细胞较多。结论异体神经干细胞大动物皮层内移植后可良好存活并分化。

Objective To research the survival and differentiation of neural stem cells being transplanted into goats＇ brain cortices. Methods We transplanted the neural stem cells marked by green fluorescence protein （GFP） or saline into the surrounding areas of brain contusion and laceration areas of goat＇s models by surgical intervention. Then, the survival and differentiation of neural stem cells being transplanted into goats＇ brain were observed one month later with the method of immunohistochemistry by detecting the specific markers of neurons, astrocytes and oligodendrocytes： microtubule-associated protein 2 （MAP2）, glial fibriUary acidic protein （GFAP） and oligodendrocyte marker protein 01 （O1）. Results Neural stem cells survived well in the goats＇ brain, and differentiated into three types of cells： neurons, astrocytes and oligodendrcytes; and these cells mainly expressed MAP2 and GFAP. Conclusion Neural stem cells can survival, immigrate and different well in the transplanted brains.