中国汉族男性人群巨噬细胞移动抑制因子-173G／C基因多态性与原发性痛风的易感性研究

Relationship between macrophage migration inhibitory factor-173G/C polymorphism and susceptibility to gout in Chinese male population

目的探讨中国汉族男性人群巨噬细胞移动抑制因子（MIF）启动子区-173G／C（rs755622）基因多态性的分布与原发性痛风遗传易感性之间的关系。方法选取380例痛风患者和378名健康对照者，采用聚合酶链反应．序列特异性引物（PCR-SSP）技术，检测中国汉族男性人群中MIF启动子区-173G／C基因等位基因及基因型频率的遗传分布，HaMy-Weinberg检验确认标本的群体代表性后，采用χ2检验进行关联分析，采用t检验对MIF基因-173G／C位点基因多态性与痛风危险因素进行比较。结果痛风组MIF启动子区-173位点GG、GC、CC基因型的频率为62．1％（236个）、34．2％（130个）、3．7％（14个）；健康对照组MIF启动子区-173位点GG、GC、CC基因型频率为66．5％（252个）、29．8％（113个）、3．7％（14个），2组之间比较差异无统计学意义（χ2=1．713，P=0．425）。痛风组等位基因G、C的频率分别为79．2％（602个）和20．8％（158个），健康对照组为81．4％（617个）和18．6％（141个），2组之间等位基因G、C频率的比较差异也无统计学意义（χ2=1．148，P=0．302）。将痛风组基因型分析为GG基因型与Gc基因型合并为GG±GC组，结果显示：GG±GC组与CC组之间年龄[（51±13）和（50±15）岁，t=0．369，P=0．712]、血糖[（7．1±8．8）和（6．1±1．2）mmol／L，t=0．352，P=0．725]、甘油三酯[（2．3±1．6）和（2．9±3.4）mmol／L，t=-1．207，P=0．228]、胆固醇[（5.3±1．2）和（5．7±1．4）mmol／L，t=-1．207，P=0．228]、尿素氮[（5．8±2．9）和（6．2±2．2）mmol／L，t=0．513，P=0．608]、肌酐[（92±52）和（84±17）μmol／L，t=0．537，P=0．592]、尿酸[（472±103）和（557±154）μmol／IJ，t=-2．949，P=0．03]比较差异均无统计学意义（均P〉0．05）、经t检验，MIF基因-173G／C基因多态性位点与痛风病危险因素之间无相关性（乃0．05o结论中国汉族男性人群中MIF基因启动子区-173G／C基因多态性与痛风易感性之间没有关联性。

Objective To explore gene polymorphism of the G/C genotype of -173G/C （rs755622） in the promoter of macrophage migration inhibitory factor （MIF） gene in male population, and thus to investigate the relationship between gene polymorphism of MIF and gout. Methods A total of 380 gout patients and 378 healthy controls were enrolled. The possible assoeiad0n between the polymorphism of MIF -173G/C and gout in Chinese were investigated and genotype frequencies itnd allelic frequencies were analyzed by polymerase chain reaction with sequence-specific primers （PCR-SSP） method. Hardy-Weinberg was used to verify the representativeness of the samples. Comparisons between the groups were performed with X2 test. The gene polymorphism of MIF and gout was performed by t test. Results The frequencies of GG, GC, CC genotypes were 62.1%（236 eases）, 34.2%（130 eases） and 3,7%（14 cases）, respectively among gout patients, while they were 66.5%（252 cases）, 29.8%（ 113 cases） and 3.7%（ 14 cases）, respectively among the controls. There was no statistical difference in MIF -173G/C genotype frequencies between gout patients and controls （χ2= 1.713, P=0.425）. The allele frequencies of G and C in gout cases were 79.2%（602 eases） and 20.8%（158 cases）, while the controls were 81.4% （617 cases） and 18.6% （141 cases）, and no significant difference between them could be found （χ2=-1.148, P=0.302）. Combine GG and GC of gout into GG＋GC, the association analysis of the two groups showed that, mean age, leves of glucose, TG, TC, BUN, Cr and UA of the GG＋GC group and the CC group were （51±13） and （50±15）t=0.369, P=0.712; （7.1±8.8） and （6.1±1.2） mmol/L, t=0.352, P=0.725; （2.3±1.6） and （2.9±3.4） mmol/L,t=-1.207, P=0.228; （5.3±1.2） and （5.7±1.4） mmol/L, t=-1.207, P=0.228; （5.8＋2.9） and （6.2±2.2） mmol/L, t=-0.513, P=-0.608; （92±52） and （84±17）μmol/L, t=0.537, P=0.592; （472±103） vs （557±154） μmol/L, t=-2.949, P=0.03 respectively; no significant difference was found in the two group. Moreover, no association between MIF -173G/C genotypes and risk factors for gout were detected in gout eases by t-test. Conclusion Results of the present study suggest that the G/C genotype of -173G/C in the promoter of MIF gene is not associated with gout in male population.