人IL-16重组腺病毒抑制MT-4细胞增殖和促进细胞凋亡

Recombinant human IL-16 inhibits proliferation and induces apoptosis in MT-4 cells

目的探讨人IL-16重组腺病毒对MT-4细胞增殖和凋亡的影响。方法用IL-16重组腺病毒感染MT-4细胞后,不同时间收获细胞。MTT法检测细胞增殖,藻红蛋白标记的annexin V和7-氨基放线菌素D联合染色(annexin V-PE/7-AAD)结合流式细胞术检测感染后MT-4细胞的凋亡、Western blot法检测MT-4细胞Bax、Bcl-2蛋白的表达。结果 MTT法结果证明IL-16腺病毒感染的MT-4细胞增殖受到明显抑制;流式细胞术检测结果表明细胞凋亡增加,Western blot法证实Bax蛋白表达量增强,而Bcl-2蛋白表达量减弱。且呈现一定程度的时间依赖性。结论人IL-16重组腺病毒可通过下调Bcl-2蛋白的表达、上调Bax蛋白的表达上调诱导MT-4细胞凋亡。

Objective To investigate the effects of recombinant human IL-16 （rhlL-16） on the proliferation and apoptosis of MT-4 cells. Methods MT-4 cells were infected with adenovirus expressing rhlL-16 at different time points. Then the proliferation of MT-4 cells was measured by the IVl-l-r assay. The apoptosis of the MT-4 cells was evaluated by flow cytometry. The expression of two molecular markers for apoptosis, Bax and Bcl-2, were analyzed by Western blotting. Results The M＇I-I- assay results showed that pAd-lL-16 transfectants had inhibitory effect on the proliferation of MT-4 cells, compared with untreated cells at various time points. Flow cytometry revealed that apoptotic rate of MT-4 cells was higher than that of control group. Furthermore, Western blotting indicated that the Bax protein expression increased while Bcl-2 protein expression decreased in a time-dependent manner in MT-4 cells infected with the rhlL-17-expressing adenovirus. Conclusion These results demonstrated that IL-16 plays an important role in the proliferation and apoptosis of MT-4 cells, and the molecular mechanism underlying this phenomenon may involve the modulation of Bax and Bcl-2 expression.