丹参酮ⅡA对Aβ_(1-42)处理的体外培养海马脑片组织神经元与胶质细胞的影响

Effect of tanshinone Ⅱ A on the expressions of NeuN,GFAP and CD11b in Aβ_(1-42) induced newborn rat hippocampal slices in vitro

目的观察丹参酮ⅡA(TanⅡA)干预Aβ1-42处理体外培养的新生大鼠海马脑片组织中神经元特异核蛋白(NeuN)、胶质纤维酸性蛋白(GFAP)和CD11b表达的影响。方法将大鼠海马脑片随机分为正常对照组、Aβ高、低剂量模型组和Aβ模型组联合TanⅡA高、低剂量组,共7组。其中正常组仍用完全培养液培养,Aβ高剂量模型组为完全培养基加5μg Aβ1-42,Aβ低剂量模型组为完全培养基加0.5μg的Aβ1-42,TanⅡA药物处理组分别为Aβ模型组加8μg或16μg TanⅡA。免疫组织化学染色法检测TanⅡA处理后各组NeuN、GFAP和CD11b表达水平的改变。结果与正常对照组比较,不同剂量Aβ处理组NeuN表达均减少,其中高剂量组下降明显(P<0.05),用高剂量TanⅡA干预后NeuN表达量均显著上升(P<0.05);对比正常对照组,Aβ处理组中GFAP和CD11b表达量均上升,其中高剂量Aβ处理组中两种蛋白表达量显著增高(P<0.05),TanⅡA处理组2种蛋白表达均下降,高剂量组下降明显(P<0.05)。结论 TanⅡA干预Aβ诱导的AD海马脑片模型,可有效的减少组织中GFAP和CD11b的表达水平,抑制胶质细胞的活化。

[ Abstract] Objective To detect the expressions of neuron-specific nuclear protein （NeuN）, glial fibrillary acidic protein （GFAP） and CDIIb in the Aβ1- 42 induced newborn rat hippocampal slices in vitro and investigate the neuroprotective effect of tanshinone ]] A. Methods Hippocampal slices were randomly divided into ？ groups： normal control group; Aβ1- 42 （5.0, 0. ,5 iJg/mL） groups; Aβ（5.0 iJg/mL） ＋ low or high （8.0 mg or 16 mg） doses of Tan HA groups,Aβ （0.5 pg/mL） ＋ low or high （8.0 mg or 16 mg） doses of Tan Ⅱ A groups. The expression levels of GFAP, NeuN and CDllb were detected by immunohistochemistry. Results Compared with the control group, the expression of NeuN decreased in the two different doses of Aβ1- 42 groups, especially in the high-dose group （P 〈 0.05 ） ; while the level significantly increased after high-dose Tan H A treatment （P〈O. 05）. Compared with the control group, the expression levels of GFAP and CDllb increased in the two different doses of Aβ1- 42 groups, and the levels in the high-dose group were the highest （ P 〈 0. 05 ） ; while the levels were reduced significantly by Tan HA treatment, the more by the high-dose TanⅡA （ P 〈0. 05）. Conclusion Tan HA can down- regulate the levels of GFAP and CDIlb and inhibit the activity of glial cells in rat hippocampal slices induced by Aβ1- 42 in vitro.