细胞凋亡在肝癌浸润转移过程中的状况

The condition of apoptosis in hepatocellular carcinoma infiltration and metastasis

目的 研究肝细胞癌（HCC)凋亡在浸润转移过程中的重要性。方法 采用DNA 原位末端标记法（ISEL）、光镜、电镜，并结合计算机图象分析技术定量检测55例HCC的凋亡发生情况。结果 低分化HCC凋亡指数低于高分化HCC （Ｐ<0.05），浸润性和转移性HCC的凋亡指数分别低于非浸润性和无转移HCC（Ｐ<0.05和Ｐ<0.01）。结论 HCC凋亡指数与肿瘤的某些生物学特性有密切的联系，细胞凋亡的异常改变可能在HCC的浸润转移过程中起重要作用。

Objective To explore the role of apoptosis in hepatocellular carcinoma(HCC) infiltration and intrahepatic metastasis. Methods Using the in situ DNA end-labeling, light microscopy,electron microscopy in combination with computer-imaging analytic technique, we tested quantitatively apoptotic cells in 55 HCC tissues. Results AI was significantly lower in poorly differentiated HCCs than well differentiated HCCs(Ｐ<0.05). Furthermore, HCCs with infiltration and intrahepatic metastasis had a significantly lower AI than those without infiltration and intrahepatic metastasis, respectively (Ｐ<0.05 and Ｐ<0.01). Conclusion AI has a close relation to some biological characteristics of HCC. The abnormal change in apoptosis may play an important role in the infiltration and intrahepatic metastasis of HCC.