HBV新型免疫原的设计、合成及免疫原性研究

Studies on molecular design, chemical synthesis and immunogenicity of novel antigenic peptides against hepatitis B virus

目的 探讨如何将表位构建成有效免疫原。方法 以Pre S( 2 ) 蛋白两优势性B细胞表位和HBcAg两Th细胞表位为基础 ,设计、合成了 2种MAP多肽 ,以此两MAP肽为免疫原免疫兔和小鼠 ,观察体液免疫反应。结果 此两种MAP多肽均比Pre S( 2 ) 蛋白诱发更高抗体滴度 ;Th细胞表位引入可显著增强反应强度。结论 增加肽抗原结构复杂性可提高其免疫原性 ,其表位排列以B细胞表位在赖氨酸核心外侧为佳 。

Objective To explore how to arrange and construct different epitope structure to obtain an effective immunogen. Methods Based on 2 dominant B cell epitopes within the Pre S (2) region of HBs Ag and 2 Th cell epitopes within HBc Ag, 2 novel antigen peptides (MAP 1 and MAP 2) were designed and synthesized. Outbred rabbit and inbred mice were immunized with these 2 novel antigens and their humoral immune response was observed to evaluate the effect of the novel antigens in animals. Results The 2 MAPs evolked antibody with higher titer than the cognate Pre S (2) protein and the control peptide in both the rabbits and mouse. The induction of Th cell epitopes enhanced the immunogenicity of MAP. Conclusion Increased the complexity of the peptide antigens may improve their immunogenicity. MAP structure is a good candidate to design a new generation of peptide based vaccines.