甲真菌病组织切片及溶甲涂片中真菌的凝集素结合形式

Study on the Lectin Binding Patterns of Fungi in Histological Section and in Nail Lysate from Patients with Onychomycosis

目的 探讨甲真菌病组织切片、溶甲涂片中真菌的凝集素结合形式，并检测其与培养中相应真菌凝集素结合形式是否等同。 方法 对已分离鉴定致病菌的 72例甲真菌病的病甲组织切片、溶甲涂片，用 ABC法对 12种凝集素进行组化染色，并与已知培养中的相应菌种进行比较。结果 44例甲组织切片、 28例溶甲涂片内 7个种、属的真菌与其培养中的相应真菌呈现相同的凝集素结合形式，仅铁锈色小孢子菌豌豆凝集素的结合形式与培养中不同，从而表明真菌表面的糖蛋白作为凝集素受体的结构颇为稳定，在经过标本制备处理后仍保持种、属的特点。溶甲涂片方法还具有阳性率高和省时的优点。结论甲组织切片、溶甲涂片内真菌的凝集素结合形式与培养中相应真菌具有相同的凝集素结合形式，有助于甲真菌病病原菌的鉴定。

Objective To study the lectin binding patterns of fungi in histological sections and in smears of sodium hydroxide lysed nail from the patients with onychomycosis. Methods Using 12 biotinyl lectins and avidin horseradish peroxidase(ABC method), the binding patterns of the fungi in the histological sections and nail lysates from 72 cases of onychomycosis were studied and compared with their counterparts in the published reports. Results All the lectin binding patterns of 7 fungi in the histological sections and nail lysates from onychomycosis patients showed identical with their counterparts in the culture, except that Microsporum ferrugineum stained positively with pisum sativum agglutinin in tissues while negatively in the culture. The nail lysate method revealed advantages of high positive rate and time saving procedure. Conclusion The lectin binding patterns of the fungi in histological sections and nail lysates appear identical with their cultured counterparts and may be helpful in mycological identification in causal fungi in onychomycosis.