细胞因子上调大肠癌细胞Fas配体表达

Cytokines upregulate Fas ligand protein expression in human colon cancer cells

目的：研究白介素 18（ IL- 18）等内源性细胞因子对大肠癌细胞 FasL表达水平的调控作用。方法：采用 Western蛋白印迹法测定细胞因子处理后大肠癌细胞的 FasL表达水平。结果：在检测的 6株大肠癌细胞中，全部表达 FasL蛋白； TNF-α和 IFN-γ显著上调 DLD- 1 FasL蛋白的表达水平； TNF-α和 IFN-γ 以及乙酸肉豆佛波醇 (phorbol 12- myristate 13- acetate,PMA)加离子霉素 (ionomycin)显著上调 SW620的 FasL蛋白表达水平，并且 TNF-α和 PMA加离子霉素处理增加 SW620的 FasL蛋白表达具有时间效应；低浓度 IL- 18增强 DLD- 1和 SW620的 FasL蛋白表达，并具有时间效应。结论： TNF－α和 IFN-γ 以及 IL- 18等细胞因子具有上调大肠癌细胞 Fas配体表达的作用。

Objective： The current study was designed to investigate regulation effect of endogeneous cytokines to Fas ligand protein expression in human colon carcinoma cell lines. Method： Six human colon cancer cell lines were examined by using Western blot analysis for Fas ligand(FasL) expression and following treatment with TNF-α , IFN-γ and IL- 18. Results： Fas ligand protein was expressed in all of these six cancer cell lines. TNF-α and IFN-γ significantly upregulated the expression of FasL protein in DLD- 1. FasL expression in SW620 was upregulated by TNF-α , IFN-γ and PMA plus ionomycin, and by TNF- alpha and PMA plus ionomycin in a time- dependent fashion. FasL expression was also upregulated by interleukin- 18 at low concentration in DLD- 1 and SW620 in a time- dependent fashion. Conclusion： These data suggest that FasL expressed in human colon cancer cells may be regulated by endogenous cytokines such as interleukin- 18, TNF-α and IFN-γ .