胀果甘草药渣总黄酮和甘草查尔酮A的制备及其体外抗肿瘤活性研究

Preparation and antitumor activities in vitro of total flavonoids from residues of Glycyrrhiza inflata and licochalcone A

目的研究胀果甘草药渣总黄酮和其指标性成分甘草查尔酮A的制备及其体外抗肿瘤活性。方法利用大孔树脂柱色谱、聚酰胺柱色谱等方法,制备甘草药渣总黄酮,并结合色谱法和波谱法分离鉴定指标性甘草查尔酮A,应用HPLC法测定了总黄酮中甘草查尔酮A。应用A549、H1792、Calu-1 3种人癌细胞系,采用MTT法和流式细胞术法系统评价甘草药渣总黄酮和甘草查尔酮A的体外抗肿瘤活性和作用机制。结果制备得到甘草药渣总黄酮,并从中分离鉴定了特征性指标性成分甘草查尔酮A,测定其在甘草药渣总黄酮中质量分数为7.41%。甘草药渣总黄酮和甘草查尔酮A对A549、H1792、Calu-1人癌细胞系均具有显著的抑制活性,可诱导肿瘤细胞凋亡。经流式细胞术检测,推测其作用机制为诱导肿瘤细胞凋亡。结论甘草查尔酮A为胀果甘草药渣总黄酮发挥体外抗肿瘤活性的有效成分。

Objective To prepare the total flavonoids and its characteristic constituent licochalcone A from the residues of Glycyrrhiza inflata,which were further assayed for the antitumor activities in vitro.Methods The total flavonoids and licochalcone A were purified by chromatography.The structure of licochalcone A was identified on the basis of spectral data.The concentration of licochalcone A was determined by HPLC analysis method.Using MTT and flow cytometry,the antitumor activities in vitro were tested against A549,H1972,and Calu-1 human cancer cell lines.Results The total flavonoids and licochalcone A were prepared respectively and the concentration of licochalcone A was determined as 7.41% in the total flavonoids.The total flavonoids and licochalcone A showed the significant antitumor activities against the above three cancer cell lines.Its possible mechanism was the induction of apoptosis.Conclusions Licochalcone A is the effective antitumor constituent of the total flavonoids from the residues of G.inflata.