正交试验优选复方太子参颗粒处方药材的提取工艺研究

Extraction technology of Compound Taizishen Granule prescription by orthogonal test

目的对复方太子参颗粒处方药材的提取工艺进行研究,优选出最佳提取工艺。方法采用紫外分光光度法测定总多糖和总皂苷,以总多糖和总皂苷提取率为综合指标,应用正交试验设计对加水量、提取时间、提取次数进行考察,确定最佳提取工艺。结果最佳提取工艺为用10倍量水提取3次,每次1.5 h。结论该提取工艺合理,可作为复方太子参颗粒处方药材的提取工艺。

Objective To study the best extracting technology of Compound Taizishen Granule prescription.Methods The total polysaccharides and total saponins were determined by UV spectrophotometer.With the content of total polysaccharides and total saponins as comprehensive indexes,orthogonal test was applied to investigation of the water addition,extraction time,and extraction times for optimizing the best extracting technology.Results The best extracting technology was using 10 times the amount of water,extracting 3 times and 1.5 h per time.Conclusion The extraction technology is safe and effective,which can be used as extraction for Compound Taizishen Granule prescription.