牛磺酸对果糖诱导的高血压胰岛素抵抗大鼠红细胞L-精氨酸/一氧化氮途径的影响

Effect of taurine on L-arginine/NO pathway in erythrocytes of rats of hypertension with insulin resistance induced by fructose

目的和方法 :在 4%果糖引起大鼠胰岛素抵抗高血压模型上 ,观察红细胞L -精氨酸 (L -Arg) /一氧化氮 (NO)途径的改变 ,并观察牛磺酸对其改变的影响。结果 :饮用果糖大鼠血压、血糖、血浆胰岛素水平明显高于对照大鼠的同时 ,红细胞对L -Arg总的转运及Y+ 载体转运的最大转运速率 (Vmax)分别低于对照大鼠31%和 37% (P <0 .0 1) ;米氏常数 (Km)分别比对照大鼠高 35 %和 30 % (P <0 .0 1)。红细胞内一氧化氮合酶(NOS)活性、cGMP水平和血浆NO含量明显低于对照大鼠 (P <0 .0 1)。应用牛磺酸治疗可明显逆转上述改变 (P<0 .0 1)。结论 :胰岛素抵抗高血压大鼠存在有红细胞L -Arg/NO系统的功能障碍 ,牛磺酸可显著提高红细胞对L -Arg的转运速率和NOS活性。

AIM and METHODS: : The present study observed the change of L-arginine (L-Arg)/Nitric oxide (NO) pathway in ergthrocytes in hypertension with insulin resistance rat induced by fructose and the effect of taurine on L-Arg/NO pathway. RESULTS: Drinking 4% fructose, while inducing blood pressure, glucose and plasma insulin contents increase, obviously decreased the maximal velocity of L-Arg transport about 31% and 37% (P<0.01), more than that of control group in total and Y+ carrier, the NO synthase (NOS) activity, nitrite (NO- 2) content and cyclic guanylate monophosphate (cGMP) level more than that of control group, but obviously enhanced Michaelis Constant (Km) about 35% and 30% (P<0.01) more than that of control group in total and Y+ carrier transport. The taurine treatment significantly counteracted the above changes. CONCLUSION: There exists a functional disturbance in L-Arg/NO system in the erythrocyte of hypertension rats with insulin resistance, but taurine can obviously enhanced the maximal velocity of L-Arg transport and NOS activity. Thus, it appears that taurine may have vital value in the treatment of hypertension with insulin resistance.