摘要
目的 :动态观察博莱霉素 (BLM)致大鼠肺纤维化过程中肺泡巨噬细胞 (AM)脂质过氧化损伤和抗氧化损伤指标的变化 ,探讨与其释放肿瘤坏死因子 (TNF -α)和血小板源生长因子 (PDGF)的关系。方法 :分离AM测定其中丙二醛 (MDA)含量和谷胱甘肽过氧化物酶 (GSH -Px)活性 ;行体外AM纯培养 ,测定其培养上清中上述两种细胞因子含量。结果 :(1)AM中MDA第 1d即明显升高 ,第 3d至高峰 ,急性期过后则逐渐降至正常 ;而GSH -Px则早期处于极低状态 ,14d后逐渐恢复近于正常。 (2 )AM释放上述两种细胞因子于第 3d开始明显增高 ,并呈动态变化。 (3)AM脂质过氧化损伤先于其释放TNF -α和PDGF。结论
AIM: To evaluate the role of alveolar macrophage(AM)lipid peroxidation in bleomycin(BLM)-induced TNF-α and PDGF release from AM in rats. METHODS: Male SD rats were intratracheally instilled with saline or BLM(5 mg/kg body weight), and AM were isolated and analyzed for malondialdehyde (MDA) and glutathione peroxidase(GSH-Px) following 1,3,7,14 or 28 day (s).Simultaneously, TNF-α and platelet-derived growth factor(PDGF) were also determined in AM-conditoned media by ELISA and bioassay respectively. RESULTS:(1) MDA in AM was markedly increased on day 1,peaked on day 3 and then decreased to near control level on day 7; on the other hand, GSH-Px activity in AM were decreased to non-detectable levels during day 1-7 after BLM instillation, returned to the control level by day 14. (2) TNF-α and PDGF were increased significantly from the third day on and then exhibited a consectutive changes.(3)AM lipid peroxidation preceded TNF-α and PDGF release. CONCLUSION: Prior oxidative changes leading to AM lipid peroxidation,depletion of antioxidative pool might be essential for the subsequent mediators release in BLM-induced pulmonary fibrosis.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2000年第11期1228-1230,共3页
Chinese Journal of Pathophysiology
关键词
肺泡巨噬细胞
脂质过氧化
肺纤维化
TNFΑ
PDGF
Macrophages
alveolar
Lipid peroxidation
Tumor necrosis factor
Platelet-derived growth factor