胃癌HER2蛋白表达及基因拷贝分析（英文）

Analysis of the Protein Expression and Gene Copy Number of HER2 in Gastric Carcinoma

目的:探讨胃癌组织HER2蛋白表达和基因拷贝数增加频率,以及蛋白表达与基因拷贝数之间的相关性。方法:分别采用免疫组织化学(IHC)和显色原位杂交(CISH)方法,检测80例青岛人胃癌组织中HER2蛋白表达和基因扩增情况。结果:HER2免疫组织化学0者51例、1+者12例、2+者12例、3+者5例。通过CISH分析:这组患者中HER2基因拷贝数扩增者共7例(8.8%),其中包括基因临界扩增3例(3.8%)。HER2的蛋白表达与基因拷贝数增加相关(P<0.05),这两个指标与肿瘤分化程度相关。HER2免疫组织化学3+和基因扩增结果一致,与乳腺癌相似。结论:这组患者中,具有较高的HER2蛋白表达和基因拷贝数增加的比率,基因扩增可能是其蛋白过表达的主要分子机制。

Objective： To investigate the frequency of the protein expression and gene copy number of HER2,and the relationship between the two markers in gastric carcinomas in Qingdao. Methods： IHC （immunohistochemistry） and CISH （Chromogenic In Situ Hy- bridization） method was used, and 80 cases protein expression and gene amplification of HER2 in gastric cancer were detected. Results： Among the 80 cases, HER2 expression was 0 in 51 tumors, I＋ in 12 tumor, 2＋ in 12 tumor and 3＋ in 5 tumor. For CISH, seven of the 80 patients （8.8%） had increased HER2 gene copy number, including 3 patients with Critical amplification （3.8%）. HER2 protein expression and genetic increased results was concordance （P〈0.05）. There was a highly significant concordance between the gene increased results and IHC 3＋ for HER2, which was similar to that of breast cancer. Conclusion： During this group, the patients had the higHer rate be- tween the protein expression and increased gene copy number; Gene amplification may be the major mechanism of HER-2 protein over- expression.