摘要
目的:检测胃癌组织中RASSF1A和Runx3基因启动子区甲基化状态,探讨二者与胃癌发生发展的关系。方法:采用甲基化特异性PCR(MSP)技术检测57例胃癌组织和相应癌旁组织及30例正常胃黏膜组织中RASSF1A和Runx3基因启动子区甲基化状态。结果:RASSF1A和Runx3甲基化在正常组未见表达。胃癌组RASSF1A基因甲基化率为64.9%(37/57),明显高于癌旁组的7.0%(4/57),差异有统计学意义(P<0.05),胃癌组Runx3基因甲基化率为49.1%(28/57),明显高于癌旁组5.3%(3/57),差异有统计学意义(P<0.05)。胃癌组RASSF1A和Runx3基因甲基化率为68.4%(39/57),明显高于癌旁组的8.8%(5/57),差异有统计学意义(P<0.05)。结论:RASSF1A和Runx3基因启动子区高甲基化与胃癌的发生密切相关,有望为胃癌的早期诊治提供理论依据。
Objective： To investigate the methylation status of the promoters of RASSF1A and Runx3 genes in the gastric cancer tissues and to assess their effect.on the tumorgenesis of gastric cancer. Methods： The aberrant methylation of RASSF1A and Runx3 genes were detected by using methylation-specific reverse transcriptase polymerase chain reaction （MSP） in gastric cancer tissues and their ad- jacent tissues from 57 patients and 30 specimens of normal. Results： Methylation of RASSF1A and Runx3 genes were not detected in normal control group. The positive rate ofRASSF1A gene methylation was significantly higher in gastric cancer tissues than in their adja- cent tissues （64.9% vs. 7.0%）. The difference was statistically significant （P〈0.05）. The positive rate ofRunx3 gene methylation was sig- nificantly higher in gastric cancer tissues than in their adjacent tissues （49.1% vs. 5.3%）. There was significant difference （P〈0.05）. The positive rates of RASSF1A and Runx3 genes methylation were significantly higher ill gastric cancer tissues than in their adjacent tissues （68.4% vs. 8.8%）. The difference was significant （P〈0.05）. Conclusion： Hypermethylation of RASSF1A and Runx3 genes are present in the gastric cancer tissues, which suggests that the hypermethylation of RASSF1A and Runx3 might be associated with the tumorigenesis of gastric cancer. They may provide helps to assistant diagnosis and treatment of gastric cancer.
出处
《现代生物医学进展》
CAS
2013年第25期4932-4935,共4页
Progress in Modern Biomedicine
基金
青岛市公共领域科技支撑计划项目(2012-1-3-1(-10)-nsh)
