高效液相色谱法测定力克舒中对乙酰氨基酚和咖啡因的含量

Determination method for paracetamol and caffeine in Likeshu capsules by HPLC

目的 :建立力克舒胶囊中对乙酰氨基酚和咖啡因的高效液相色谱测定方法。方法 :采用CLC ODSC18柱 ,甲醇 0 .0 5mol·L-1磷酸二氢钾溶液 三乙胺 (2 0∶80∶0 .2 ,磷酸调 pH3.2 )为流动相 ,检测波长 2 10nm ,流速为 1.0ml·min-1。结果 :对乙酰氨基酚线性范围为 2 4～ 2 40mg·L-1,平均回收率为 99.6 % ,RSD为 0 .8% (n =5 ) ;咖啡因线性范围为 2～ 16mg·L-1,平均回收率为 99.2 % ,RSD为 2 .2 % (n =5 )。结论 :方法简便 ,结果准确 ,可用于本制剂的含量测定和质量控制。

OBJECTIVE:To establish an assay method for paracetamol and caffeine in Likeshu capsules with HPLC. METHODS:CLC ODS C 18 was packed in the analytical column, The mobile phase consisted of methanol 0.05 mol·L -1 KH 2PO 4 triethylamine (20∶80∶ 0.2 , adjust to pH 3.2 with H 3PO 4)and the flow rate was 1.0 ml·min -1 .The UV detection wavelength was 210 nm. RESULTS:The standard curve of paracetamol was linear over the range of 24～240 mg·L -1 .The average recovery was 99.6% with RSD 0.8% (n=5) .The standard curve of caffeine was linear in the range of 2～16 mg·L -1 .The average recovery was 99.2% with RSD 2.2% (n=5) .CONCLUSIONS:This method was found to be simple and accurate. The experiments will supply reference methods for analysis of concentration and quality control. [