HPLC法测定复方珍珠暗疮片中大黄所含5种蒽醌苷元

Determination of Anthraquinone Aglycones of Radix et Rhizome Rhei in Fufangzhuenzhuanchuang Tablets by HPLC

建立复方珍珠暗疮片中大黄所含5种蒽醌苷元的含量测定方法。方法:采用高效液相色谱法,色谱条件为XBP-C18柱,流动相为乙腈-0.1%磷酸水溶液梯度洗脱,流速为1.0mL·min-1,检测波长为226nm。结果:在一定质量范围内,5种蒽醌苷元的进样量与峰面积积分值线性关系良好;5种蒽醌苷元的平均回收率(n=6)分别为95.89%、96.11%、99.21%、98.38%、96.12%,RSD分别为1.92%、1.59%、1.02%、1.13%、2.18%。结论:该法简便、快速、准确可靠、专属性强,可用于复方珍珠暗疮片的质量控制。

Objective：To determine Radix et Rhizoma Rhici in Fufangzhuenzhuanchuang tablets （Radix et Rhizoma Rhei ）. Meth- ods ： HPLC was used to simultaneously determine the content of paeonol and paeoniflorin. The method was condncted on Agela Ve nusil XBP-C18 （4.6mm×250mm, 5μm） of acetonitrile and 0.1 % phosphoric acid with gradient elution. The flow rate was 1.0mL · min -1 and the detection wavelength was 226nm. Results：The linear ranges of aloe--emodin, rhein, emodin, chrysophanol and phy scione were 0. 040～0. 800, 0. 080～1. 600, 0. 040～0. 800, 0. 040～0. 800, 0. 020～0. 400μg respectively, The average recoveries （n=6） 95.89 %0 （RSD=1. 92 %）, 96.11 % （RSD=1. 59 %）, 99.21 % （RSD=I.02 %）, 98.38% （RSD=1. 13 0%）, 96.12 （RSD= 2.18% ）, respectively. Conclusion：The method is rapid, simple and accurate, it has a good specificity and it can be suitable for the determination of twelve active components in Fufangzhuenzhuanchuang tablets simultaneously.