慢性肾功能衰竭患者与HLA-DRB1等位基因相关性分析

The association of HLA-DRB1 alleles in patients with chronic renal failure from northern Chinese

目的 :探讨东北地区汉族人HLA DRB1等位基因与慢性肾功能衰竭的遗传关联。方法 :应用聚合酶链反应———序列特异性引物 (PCR SSP)分型技术 ,对 44例慢性肾功能衰竭患者以及 336例正常人HLA DRB1等位基因进行分析。结果 :慢性肾功能衰竭患者DRB1 0 70 1的基因频率较正常对照组明显增高 ,且有非常显著性差异 (P <0 0 0 1,RR =4 76 ,Pc <0 0 1) ;患者组的DRB1 12 0 1、12等位基因频率明显高于正常对照组 ,差异有显著性 (P <0 0 0 5 ,RR =6 83 ,Pc <0 0 5 ) ;结论 :DRB1 0 70 1和DRB1 12 0 1 12 0 2基因与慢性肾功能衰竭有一定的关联 ,慢性肾功能衰竭患者与正常人之间存在着免疫遗传异质性的差异。或者可以提示携带DRB1 0 70 1和RDB1 12 0 1 12 0 2基因肾病患者 ,预后较差 ,易发展成为不可逆的慢性肾功能衰竭。

Objective:To further evaluate the nature of HLA DRB1 allelos association with chronic renal failure(CRF) from northern Chinese.Methods:HLA DRB1 typings of 44 patients with CRF and 336 control persons from northern Chinese were investigated by polymerase chain reaction sequence specific primer method,and the frequencies of DRB1 alleles were compared.Results:It was found that the patients with CRV had very significantly increased frequencies of the HLA DRB1 *0701 gene (P<0.01,RR=4.76,Pc<0.01),and DRB1 *1201/1202 gene frequency of CRF patients group was higher than that of healthy group significantly(P<0.005,RR=6.83,Pc<0.05).Conclusion:The results suggest that HLA DRB1 *0701 and DRB1 *1201/1202 genes contribute to genetic sussceptibility to chronic renal failure.