HPLC法测定复方托吡卡胺滴眼液中苯扎氯铵的含量

Determination of benzalkonium chloride in compound tropicamide eye drops by HPLC

目的:建立超高效液相色谱法测定复方托吡卡胺滴眼液中苯扎氯铵的含量测定方法。方法:色谱柱为Alltech Alltima-CN(250cm×4.6mm,5μm),流动相为0.1mol·L-1醋酸钠溶液(用冰醋酸调节pH值至5.0)-乙腈(60:40),检测波长254nm,流速为2.0ml·min-1,柱温30℃,进样体积50μL。结果:苯扎氯铵在0.02至0.5mg·mL-1浓度范围内线性关系良好(r=0.9999);平均回收率为98.91%,RSD=0.44%(n=9)。结论:该方法准确、简便、快速、可靠,能有效控制复方托吡卡胺滴眼液中苯扎氯铵的含量。

Objective ：To establish an HPLC method for detecting the content of Benzalkonium Chloride in Compound Tropicamide Eye Drops .Methods ：A Alltech Alltima -CN column （250cm × 4 .6mm ,5μm） was used ,and the mobile phase was 0 .1mol·L -1 NaAc solution -acetonitrile （60 ：40） ,HAc was used to adjust pH to 5 ,column temperature was 30℃ ,at a flow rate of 2 .0ml·min -1 and detection wavelength of 254 nm .Results ：The linear range of Benzalkonium Chloride was 0 .02-0 .5mg·mL -1 ;The correlation coefficient was 0 .9999 .The average recovery of Benzalkonium Chloride was 98 .91% （RSD=0 .44% ,n=9 ） .Conclusion ：The method is accurate ,simple ,rapid and suitable for the quality control of Benzalkonium Chloride in Compound Tropicamide eye drops .