HPLC法测定19个人参新炮制品-黑参样品中的人参皂苷含量

Simultaneous Quantification of Ginsenosides in 19 Black Ginseng Samples Developed grom Panax Ginseng by HPLC

目的:建立黑参炮制工艺标准和质量控制标准。方法:HPLC法测定19个人参新炮制品-黑参样品中的人参皂苷含量。结果:人参皂苷Rg1、Re、Rb1在MSG80A180系列中的含量普遍高于MSG90A360(1)。在MSG80A180系列样品中,人参皂苷Rg1、Re、Rb1含量先增加后减少。在MSG90A180系列中,人参皂苷Rg1、Re、Rb1含量先降低后升高。在MSG110B120系列样品中,人参皂苷Rg1和Re含量先减少后增加,而Rb1正好相反。在MSG121B060系列样品中,Rg1呈现先增加后减少的趋势,而Rb1和Re呈现增加-减少-增加的趋势。在MSG121B120系列样品中,3种人参皂苷含量降低得很快,Rg1和Re几乎检测不到。BGKBRG样品中不含这3种人参皂苷成分,RGKINH样品中含有Rg1和Rb1,但含量很低。显然,成分变化在这些样品中没有呈现连续性,说明温度、压力和时间都对黑参炮制工艺有影响。而且,通过横向比较,更多的峰数和更高的峰值产生在无压力的样品中。随着蒸制次数的增加,这3种人参皂苷成分应变化成其他皂苷成分。结论:温度、压力和时间对黑参制作工艺有影响。有必要对黑参的炮制进一步深入研究。同时,在以后的研究中,应通过寻找黑参独有的标志性成分来建立和完善其炮制工艺标准和质量控制标准。

Objective：This experimental study was performed to establish manufacture process standard and quality control standard of black ginseng. Methods ： Ginsenosides contents in 19 black ginseng samples were simultaneously determined by HPLC. Results：The contents of ginsenosides Rgj, Re, Rb1 in MSGSOA180 series, were more than that in MSG90A360 （1）. Among these samples of MSGSOA180 series,the contents of ginsenosides Rgl ,Re, Rbl were all firstly increased and then decreased. The con- tents of ginsenosides R1, , Re, Rbl in MSG90A180 series were firstlv declined and then increased. In MSG110B120 series, thecontents of ginsenoside Rg1 and Re were firstly decreased and then increased, but ginsenoside Rbl was on the contrary. Ginsen- oside Rg1 was in a rise - decline trend, while ginsenoside RbI and Re were in a rise - decline - rise trend in MSG121B060 series. In MSG121BI20 series,three kinds of ginsenosides were decreased rapidly,Rg1 and Re were almost not detected. Company A＇s sample BGKBRG did not contain ginsenosides RgI ,Re and Rbl. RGKINH did not contain Re but Rg1 and RbI ,and the contents of Rgl and Rbl were relatively low. Obviously, content change didnt show a consistent trend, so temperature, pressure and time all had effects on standard manufacture process of black ginseng. In addition, horizontal comparison, more peak numbers and higher peak value were in no pressure samples. According to the pharmacopoeia criterion,only MSG80A180（5 ） complied with it. With steaming more times, ginsenosides may change into other components. Conclusion：Some factors including temperature, pressure and time all have eftects on standard manufacture process of black ginseng. It＇s necessary to further study on the production of black ginseng. Moreover,in later experiments, we should focus on other ginsenosides to find out the unique and index components to establish the standard of black ginseng.