骨质疏松及其骨量变化与弓状核的关系

Interrelation between the hypothalamic arcuate nucleus and the bone variation of osteoporosis in rats

本文旨在研究大鼠骨质疏松及其骨量变化与弓状核的关系。用去卵巢大鼠骨质疏松、糖皮质激素性骨质疏松、维甲酸诱发骨质疏松和老年性骨质疏松4种典型骨质疏松动物模型,环孢素、胸腺肽和运动二种干预骨质疏松的实验,研究骨质疏松及其骨量变化与弓状核的关系。结果表明:(1)4种典型的骨质疏松都伴有弓状核神经细胞数量减少;(2)胸腺肽和运动使骨质疏松骨量丢失减轻,并伴有弓状核神经细胞数量增加;环孢素使骨质疏松骨量丢失加重,并伴有弓状核神经细胞数量减少;骨量丢失的程度与弓状核神经细胞数量密切相关。结果提示:大鼠骨质疏松及其骨量变化与弓状核神经细胞数量密切相关;存在下丘脑—垂体—骨轴;骨质疏松受下丘脑—垂体—骨轴三级水平调控。

This study investigated the interrelation between the hypothalamic arcuate nucleus （ARC） and the bone variation of osteo- porosis in rats. Four animal models of ovariectomy-induced osteoporosis （OVX）, glucocorticoid-induced osteoporosis （GIOP）, retino- ic acid-induced osteoporosis （RAOP） and senile osteoporosis （SOP） were used to study the influence of osteoporosis on ARC. Ova- riectomized rats were further treated with thymopeptide, ciclosporin and exercise respectively to detect the changes of ARC nerve cells number. The results showed that： （1） The OVX, GIOP, RAOP and SOP models were successfully established, showing osteopo- rosis as well as decrease of the number of ARC nerve cells; （2） Thymopeptide and exercise respectively alleviated the bone loss induced by ovariectomy, accompanied by increase of the number of ARC nerve cells, while ciclosporin further increased the bone loss of ovariectomized rats, accompanied by further decrease of the number of ARC nerve cells. These results suggest that there is a close interrelationship between ARC and osteoporosis in rat. There is a hypothalamus-pituitary-bone （HPB） axis, and HPB axis regulates the mechanism of osteoporosis in rats.