摘要
目的观察蛋白酪氨酸磷酸酶1B(protein tyrosine phosphatase 1B,PTP1B)在胃癌中的表达及其临床意义。方法采用qRT-PCT和Western blot法检测胃癌细胞中PTP1B mRNA和蛋白的表达;采用免疫组化SP法检测80例胃癌组织及癌旁正常组织中PTP1B蛋白表达并分析PTP1B表达与胃癌各临床病理特征的关系。结果 PTP1B在不同胃癌细胞和胃癌癌组织中呈不同程度的阳性表达,在永生化胃黏膜细胞GES-1和癌旁正常组织中弱表达或不表达。PTP1B表达与胃癌TNM分期、淋巴结转移及肿瘤在原发部位的浸润显著相关(P<0.05),PTP1B在TNM分期较晚(Ⅲ+Ⅳ)和有淋巴结转移(N1+N2+N3)的胃癌组织中的表达明显高于其在TNM分期较早(Ⅰ+Ⅱ)和无淋巴结转移(N0)的胃癌组织。结论 PTP1B在胃癌发生、发展过程中可能起到类似原癌基因的作用,其有望成为胃癌治疗的潜在靶点。
Purpose To investigate the expression of PTP1B in gastric cancer and to determine the association between PTP1B expres- sion and the development of gastric cancer. Methods The expression of PTP1B in 6 difference gastric cancer cell lines and immortal- ized gastric mucosal epithelial cell line (GES-1) was examined by RT-PCR and Western blot. The gastric cancer tissues from 80 pa- tients were collected from July 2008 to May 2009 in Ruijin Hospital, Shanghai Jiao Tong University School of Medicine. 80 cases of the adjacent normal gastric tissues were enrolled as controls. The expression of PTP1B was detected by immunohistochemical staining. Then the relationship between the expression of PTP1B and clinicopathologic parameters of gastric cancer was analyzed. Results The expression of PTPI B in all 6 gastric cancer cell lines was significantly up-regulated than that in GES-1. in addition, the expression of PTP1B was shown moderate or more positive staining in gastric cancer tissues but not in adjacent normal gastric tissues. The expression of PTP1B was significantly correlated with the TNM stage, lymph node metastasis and tumor stage of gastric cancer. The expression lev- el of PTP1B in gastric cancer with lately TNM stage ( Ⅲ + Ⅳ ) and lymph node invasion ( N1 + N2 + N3 ) was significantly higher than that with early TNM stage ( Ⅰ + Ⅰ) and without lymph node invasion (N0). Conclusion PTPIB might play an oncogene role in the development of gastric cancer and be a potential therapy target for gastric cancer.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2013年第10期1051-1055,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
安徽高校省级科学研究项目(KJ2011B193)
皖南医学院弋矶山医院引进人才科研基金(YR201002)
芜湖市科技计划(2012hm29-1)
关键词
胃肿瘤
蛋白酪氨酸磷酸酶
PTP1B蛋白
肿瘤转移
stomach neoplasms
protein tyrosine phosphatases
PTP1B protein
neoplasm metastasis