期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

胃癌中酪氨酸磷酸酶1B的表达及临床意义 被引量：1

Expression of protein tyrosine phosphatase 1B and its clinical significance in gastric cancer
下载PDF
导出
摘要 目的观察蛋白酪氨酸磷酸酶1B(protein tyrosine phosphatase 1B,PTP1B)在胃癌中的表达及其临床意义。方法采用qRT-PCT和Western blot法检测胃癌细胞中PTP1B mRNA和蛋白的表达;采用免疫组化SP法检测80例胃癌组织及癌旁正常组织中PTP1B蛋白表达并分析PTP1B表达与胃癌各临床病理特征的关系。结果 PTP1B在不同胃癌细胞和胃癌癌组织中呈不同程度的阳性表达,在永生化胃黏膜细胞GES-1和癌旁正常组织中弱表达或不表达。PTP1B表达与胃癌TNM分期、淋巴结转移及肿瘤在原发部位的浸润显著相关(P<0.05),PTP1B在TNM分期较晚(Ⅲ+Ⅳ)和有淋巴结转移(N1+N2+N3)的胃癌组织中的表达明显高于其在TNM分期较早(Ⅰ+Ⅱ)和无淋巴结转移(N0)的胃癌组织。结论 PTP1B在胃癌发生、发展过程中可能起到类似原癌基因的作用,其有望成为胃癌治疗的潜在靶点。 Purpose To investigate the expression of PTP1B in gastric cancer and to determine the association between PTP1B expres- sion and the development of gastric cancer. Methods The expression of PTP1B in 6 difference gastric cancer cell lines and immortal- ized gastric mucosal epithelial cell line （GES-1） was examined by RT-PCR and Western blot. The gastric cancer tissues from 80 pa- tients were collected from July 2008 to May 2009 in Ruijin Hospital, Shanghai Jiao Tong University School of Medicine. 80 cases of the adjacent normal gastric tissues were enrolled as controls. The expression of PTP1B was detected by immunohistochemical staining. Then the relationship between the expression of PTP1B and clinicopathologic parameters of gastric cancer was analyzed. Results The expression of PTPI B in all 6 gastric cancer cell lines was significantly up-regulated than that in GES-1. in addition, the expression of PTP1B was shown moderate or more positive staining in gastric cancer tissues but not in adjacent normal gastric tissues. The expression of PTP1B was significantly correlated with the TNM stage, lymph node metastasis and tumor stage of gastric cancer. The expression lev- el of PTP1B in gastric cancer with lately TNM stage （ Ⅲ ＋ Ⅳ ） and lymph node invasion （ N1 ＋ N2 ＋ N3 ） was significantly higher than that with early TNM stage （ Ⅰ ＋ Ⅰ） and without lymph node invasion （N0）. Conclusion PTPIB might play an oncogene role in the development of gastric cancer and be a potential therapy target for gastric cancer.
作者 汪进国 陈雪华 刘炳亚 朱正纲
机构地区 皖南医学院弋矶山医院胃肠外科 上海交通大学医学院附属瑞金医院普外科
出处 《临床与实验病理学杂志》 CAS CSCD 北大核心 2013年第10期1051-1055,共5页 Chinese Journal of Clinical and Experimental Pathology
基金 安徽高校省级科学研究项目(KJ2011B193) 皖南医学院弋矶山医院引进人才科研基金(YR201002) 芜湖市科技计划(2012hm29-1)
关键词 胃肿瘤 蛋白酪氨酸磷酸酶 PTP1B蛋白 肿瘤转移 stomach neoplasms protein tyrosine phosphatases PTP1B protein neoplasm metastasis
分类号 R735.2 [医药卫生—肿瘤]
  • 相关文献

参考文献7

  • 1Yip S C, Saha S, Chernoff J. PTP1B: a double agent in metabo- lism and oncogenesis [ J ]Trends Biochem Sci, 2010,35 ( 8 ) : 442 -9.
  • 2Aceto N, Bentires-Alj M. Targeting protein-tyrosine phosphatases in breast cancer [ J ]. Oncotarget, 2012,3 ( 5 ) :514 - 5.
  • 3Zhu S, Bjorge J D, Fujita D J. PTP1B contributes to the oncogen- ic properties of colon cancer cells through Src activation [ J ]. Cancer Res, 2007,67 (21) : 10129-37.
  • 4李蓓蓓,李亚玲,王学斌,宋明芮,张煦.结直肠癌中NKD1和β-catenin的表达及意义[J].临床与实验病理学杂志,2013,29(4):381-384. 被引量：10
  • 5Zheng L Y, Zhou D X, Lu J, et al. Down-regulated expression of the protein-tyrosine phosphatase 1B (PTP1 B) is associated with ag- gressive clinicopathologic features and poor prognosis in hepatocel- lular carcinoma [ J]. Biochem Biophys Res Commun, 2012,420 (3) :680 -4.
  • 6Balavenkatraman K K, Aceto N, Britsehgi A, et al. Epithelialprotein-tyrosine phosphatase 1B contributes to the induction of mammary tumors by HER2/Neu but is not essential for tumor main- tenance [ J ]. Mol Cancer Res, 2011,9 (10) : 1377 - 84.
  • 7Lessard L, Labb6 D P, Deblois G, et al. PTP1B is an androgen receptor-regulated phosphatase that promotes the progression of prostate canccr[ J]. Cancer Res, 2012,72 (6) : 1529 - 37.

二级参考文献12

  • 1Maruyama K, Ochiai A, Akimoto S, et al. Cytoplasmic beta-cate- nin accumulation as a predictor of hematogenous metastasis in hu- man colorectal cancer [ J ]. Oncology, 2000,59 (4) :302 - 9.
  • 2Wahher A, Johnstone E, Swanton C, et al. Genetic prognostic and predictive markers in colorectal cancer[ J ]. Nat Rev Cancer, 2009,9 (7) :489 - 99.
  • 3Bienz M, Clevers H. Linking colorectal cancer to Wnt signaling [J]. Cell, 2000,103(2) :311 -20.
  • 4Price M A. CKI, there' s more than one: casein kinase I family members in Wnt and Hedgehog signaling[ J]. Genes Dev, 2006, 20(4) :399 -410.
  • 5Firestein R, Hahn W C. Revving the Throttle on an oncogene: CDK8 takes the driver seat[J]. Cancer Res, 2009,69(20) :7899 - 901.
  • 6Wharton K A Jr, Zimmermann G, Rousset R, Scott M P. Verte- brate proteins related to Drosophila Naked Cuticle bind Dishevelled and antagonize Wnt signaling[J]. Dev Biol, 2001,234( 1 ) :93 - 106.
  • 7Yan D, Wallingford J B, Sun T Q, et al. Cell autonomous regula- tion of multiple Dishevelled-dependent pathways by mammalian Nkd[J]. Proe Nail Acad Sei USA, 2001,98(7) : 3802 -7.
  • 8Rousset R, Mack J A, Wharton K A Jr, et al. Naked cuticle tar- gets dishevelled to antagonize Wnt signal transduetion [ J ]. Genes Dev, 2001,15(6) :658 -71.
  • 9Rousset R, Wharton K A Jr, Zimmermanu G, Scott M P. Zinc- dependent interaction between dishevelled and the Drosophila Wnt antagonist naked cuticle [J]. J Biol Chem, 2002,277 (50) :49019 - 26.
  • 10Guo J, Cagatay T, Zhou G, et al. Mutations in the human naked cuticle homolog NKD1 found in colorectal cancer alter Wnt/Dvl/ beta-catenin signaling [ J ]. PLoS One, 2009,4 ( 11 ) : e7982.

共引文献9

同被引文献11

  • 1Lessard L, Stuible M, Tremblay M L. The two faces of PTPI B in cancer[J]. Biochim Biophys Acta, 2010,1804(3) :613 -9.
  • 2Krishnan N, Koveal D, Miller D H, et al. Targeting the disordered C terminus of PTP1B with an allosteric inhibitor[J]. Nat Chem Bi- ol, 2014,10(7) :558 -66.
  • 3Arias-Romero L E, Saha S, Villamar-Cruz O, et al. Activation of Src by protein tyrosine phosphatase 1B is required for ErbB2 trans-formation of human breast epithelial cells[ J]. Cancer Res, 2009, 69 ( 11 ) :4582 - 8.
  • 4Aceto N, Bentires-Alj M. Targeting protein-tyrosine phosphatases in breast cancer[J]. Oneotarget, 2012,3(5) :514 -5.
  • 5Henderson T O, Amsterdam A, Bhatia S, et al. Systematic re- view : surveillance for breast cancer in women treated with chest ra- diation for childhood, adolescent, or young adult cancer[ J ]. Ann Intern Med, 2010,152(7) :444 -55.
  • 6Revuelta-Cervantes J, Mayoral R, Miranda S, et al. Protein tyro- sine phosphatase 1B ( PTPI B ) deficiency accelerates hepatic re- generation in mice[J]. Am J Pathol, 2011,178(4) :1591 -604.
  • 7Yip S C, Saha S, Chemoff J. PTP1B: a double agent in metabo- lism and oncogenesis[ J]. Trends Bioehem Sci, 2010,35 (8) :442 --9.
  • 8Chen Q, Li Y, Li Z, et al. Overexpression of PTPI B in human colorectal cancer and its association with tumor progression and prognosis[J]. J Mol Histol, 2014,45(2):153-9.
  • 9Lessard L, Labb6 D P, Deblois G, et al. PTPIB is an androgen receptor-regulated phosphatase that promotes the progression of prostate cancer [ J ]. Cancer Res, 2012,72 ( 6 ) : 1529 - 37.
  • 10Fan G, Lin G, Lucito R, Tonks N K. Protein-tyrosine phospha- tase 1 B antagonized signaling by insulin-like growth factor-I recep- tor and kinase BRK/PTK6 in ovarian cancer cells [ J ]. J Biol Chem, 2013,288(34) :24923 -34.

引证文献1

二级引证文献1

临床与实验病理学杂志
2013年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部