摘要
目的:探讨2,3,5,4’-四羟基二苯乙烯-2-o-β-D-葡萄糖苷(2,3,5,4’-tetrahydroxystibene-2-o-β-D-glucoside,TSG)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-pheyl-1,2,3,6-tetrahydrophridine,MPTP)诱导的帕金森病(Parkinson’s disease,PD)小鼠黑质多巴胺能神经元保护作用的机制。方法:实验分成三组,即健康C57BL小鼠随机分为对照组(腹腔注射生理盐水);模型组,连续7 d给C57BL小鼠腹腔注射MPTP(30 mg/kg)制备亚急性PD模型;治疗组,在给予MPTP后1 h腹腔注射TSG(60 mg/kg)。爬杆实验检测小鼠的行为学变化;活性氧(reactive oxygen species,ROS)检测试剂盒检测ROS水平;免疫荧光组织化学染色法测定黑质酪氨酸羟化酶(TH)和多巴胺转运体(DAT)的表达变化。结果:行为学:与对照组比较,模型组爬杆时间与转头时间明显延长(P<0.01),而治疗组较模型组时间缩短(P<0.05)。模型组ROS水平较对照组大幅度升高(P<0.01),治疗组较模型组明显下降(P<0.05)。免疫荧光染色:与对照组相比,模型组TH及DAT阳性神经元的数目明显减少(P<0.01),而治疗组TH及DAT阳性神经元数目减少的幅度明显有所下降(P<0.05)。结论:TSG对MPTP诱导的神经毒性具有保护作用,其机制可能与TSG抑制ROS有关。
Objective: To investigate the mechanism of 2,3,5,4'-tetrahydroxystibene-2-o-13-D-glucoside (TSG) on do- paminergic neuron protection in substantia nigra pars compacta (SNpc) from MPTP (1-methyl-4-pheyl-1,2,3, 6-tetrahy- drophridine) induced injury in Parkinson's disease (PD) mice. Methods: C57BL mice were randomly divided into three groups. Control group were established by intraperitoneal injection of saline. Subacute PD model group were established by intraperitoneal injection of MPTP (30 mg/kg) for 7 consecutive days. The treatment group was established by intraper- itoneal injection of TSG (60 mg/kg)1 h after MPTP. The behavior changes of mice were observed by pole test. The reac- tive oxygen species (ROS) was mesured by ROS assay kit. The tyrosine hydroxylase (TH) and dopamine transpoter (DAT) positive cells were detected by immunofluorescence histochemical method. Results: Behavioral results showed that compared with control group, the time of mice climb to the bottom of the pole and turn around increased obviously (P 〈 0.01 ), while the time of TSG pretreatment group decreased compared with MPTP group ( P 〈 0.05 ). ROS level in MPTP group increased significantly compared with control group (P 〈 0.01 ), TSG pretreatment group decreased obvious- ly compared with MPTP group ( P 〈 0.05 ). Immunofluorescence histochemical staining results showed that the number of TH-and DAT-positive neurons decreased significantly compared with control group ( P 〈 0.01 ), but the reduction rate in TSG pretreatment group was lower (P 〈 0.05 ). Conclusion: TSG attenuates MPTP-induced neurotoxicity by the inhibi tion of ROS.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2013年第5期531-536,共6页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(30772743)