APP/PS1转基因小鼠发育过程中神经细胞增殖变化

The change of neural proliferation in developing APP/PS1 transgenic mouse

目的:探讨β-淀粉样前体蛋白基因(β-amyloid precusor protein,APP)和突变早老素1基因(presinilin,PS1)转基因小鼠(APP/PS1)发育过程中齿状回神经细胞增殖规律。方法:取不同发育时间(P14、P30、P180、P360)APP/PS1转基因模型鼠与同时间点对照鼠,用二等分Y型迷宫箱测试小鼠学习记忆能力,BrdU免疫细胞化学观察齿状回内神经细胞增殖变化。结果:随着小鼠的生长发育,BrdU阳性细胞密度逐渐降低,在P360时间点,模型组齿状回BrdU阳性细胞密度比对照组低(P<0.05);其空间识别以及记忆能力明显低于对照组(P<0.01)。结论:发育中的APP/PS1转基因小鼠齿状回内神经细胞增殖减少可能与阿尔茨海默病的学习、记忆能力下降有关。

Objective： To observe the neuroproliferation in the dentate gyrus of developing APP/PS1 transgenic mice. Methods： With wild type and APP/PS1 transgenic mice from postnatal day 14 to postnatal day 360（ P14 ,P30 ,P180 and P360）, two equally divided Y type electric mazing test was used to examine learning and memory ability, and immunoflourescence method with bromodeoxyuridine （BrdU） was applied to observe the changes of neuroproliferation. Results： The density of the positive BrdU cells decreased gradully along with development. At P360, the density of the positive Br- dU cells in model group was obviously lower than that in the control group （ P 〈 0.05 ） , and that spatial recognition and memory capacity was significantly lower than that in the control group （P 〈0.01 ）. Conclusion： The decreased learning and memory ability of Alzheimer＇s disease may be relevant to the decreased neuroproliferation of the dentate gyms in de- veloping APP/PS1 transgenic mice.