摘要
目的:探讨β-淀粉样前体蛋白基因(β-amyloid precusor protein,APP)和突变早老素1基因(presinilin,PS1)转基因小鼠(APP/PS1)发育过程中齿状回神经细胞增殖规律。方法:取不同发育时间(P14、P30、P180、P360)APP/PS1转基因模型鼠与同时间点对照鼠,用二等分Y型迷宫箱测试小鼠学习记忆能力,BrdU免疫细胞化学观察齿状回内神经细胞增殖变化。结果:随着小鼠的生长发育,BrdU阳性细胞密度逐渐降低,在P360时间点,模型组齿状回BrdU阳性细胞密度比对照组低(P<0.05);其空间识别以及记忆能力明显低于对照组(P<0.01)。结论:发育中的APP/PS1转基因小鼠齿状回内神经细胞增殖减少可能与阿尔茨海默病的学习、记忆能力下降有关。
Objective: To observe the neuroproliferation in the dentate gyrus of developing APP/PS1 transgenic mice. Methods: With wild type and APP/PS1 transgenic mice from postnatal day 14 to postnatal day 360( P14 ,P30 ,P180 and P360), two equally divided Y type electric mazing test was used to examine learning and memory ability, and immunoflourescence method with bromodeoxyuridine (BrdU) was applied to observe the changes of neuroproliferation. Results: The density of the positive BrdU cells decreased gradully along with development. At P360, the density of the positive Br- dU cells in model group was obviously lower than that in the control group ( P 〈 0.05 ) , and that spatial recognition and memory capacity was significantly lower than that in the control group (P 〈0.01 ). Conclusion: The decreased learning and memory ability of Alzheimer's disease may be relevant to the decreased neuroproliferation of the dentate gyms in de- veloping APP/PS1 transgenic mice.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2013年第5期577-580,共4页
Chinese Journal of Neuroanatomy
关键词
阿尔茨海默病
齿状回
神经细胞增殖
转基因小鼠
Alzheimer's disease
dentate gyms
neural proliferation
APP/PS1 transgenie mouse