细胞外基质蛋白微图形对成骨细胞黏附与铺展的影响

Effects of Extracellular Matrix Protein Micropatterns on Osteoblast Cell Adhesion and Spreading

本文的主要目的是研究大鼠原代成骨细胞在细胞外基质蛋白微图形上的生长行为。为此,采用微接触印刷术在聚苯乙烯材料表面制备了一系列具有不同形状和不同尺度的纤连蛋白微图形,激光共聚焦扫描显微镜观察结果表明,微图形形态规整,微图形在细胞培养过程中较为稳定。细胞实验表明,细胞优先黏附在蛋白区域,在不同的微图形表面表现出不同的细胞形态与铺展行为,蛋白微图形对细胞的黏附、铺展、排列以及细胞生长方向等行为具有非常明显的影响。因此,通过在材料表面构建细胞外基质蛋白微图形对生物材料特别是骨替代材料进行表面改性,可以有效地调控细胞的生长行为,有利于进一步研究材料与成骨细胞相互作用的界面生物学行为,为发展具有能够自主调控成骨细胞生长行为的骨替代植入材料提供实验依据。

The main purpose of the present study was to investigate the cell behaviors of rat primary osteoblast cells on extracellular matrix （ECM） protein micropatterns. For this purpose, a series of fibronectin micropatterns with different shapes and varying dimensions were created on polystyrene （PS） surfaces by microcontact printing. The re suits of confoeal laser scanning microscopy （CLSM） images indicated that excellent micropatterns were successfully obtained. These protein patterns were stable during the cell culture. The cell experiments suggested that the osteo- blast cells preferentially attached onto proteiwfunctionalized areas and displayed different cell shape and spreading be- havior on different protein micropatterns. The protein micropatterns can significantly influence the cell adhesion, spreading, alignment and orientation and so on. Therefore, this work can be used to modify biomaterial surfaces, es- pecially that of bone-implant biomaterials, to effectively control cell behavior. It further contributes to clarify the in- terfaeial biological behaviors between biomaterials and osteoblast and can provide the cues for development of bone implantable materials which is able to modulate osteoblast cell growth behavior.